Early interactions of Entamoeba histolytica trophozoites with parenchymal and inflammatory cells in the hamster liver: an immunocytochemical study

Ventura-Juárez, J; Campos-Rodríguez, R; Tsutsumi, V
February 2002
Canadian Journal of Microbiology;Feb2002, Vol. 48 Issue 2, p123
Academic Journal
We studied the early in situ interactions of live and fixed Entamoeba histolytica trophozoites with hamster hepatic parenchymal and inflammatory cells using immunoperoxidase and immunoelectronmicroscopy. Close contact between trophozoites and endothelial cells and the diffusion of amoebic molecules from trophozoites towards nearby endothelial cells and distant hepatocytes were observed. The inflammatory cells around the amoebae and the remnants of parenchymal cells and hepatocytes located close to the lesion had a positive stain for amoebic molecules. In the amoebae, at the ultrastructural level, molecules were attached to the membranes and inside the vesicles. These molecules were apparently released into the space formed between the parasite and the endothelial cells. The endothelial cells and the nearby and distant hepatocytes captured amoebic molecules, and later they became necrotic. Contrarily, when fixed amoebae were inoculated, amoebic molecules were captured by endothelial cells and polymorphonuclear (PMN) leukocytes, but neither suffered any damage. In this work, we are presenting evidence clearly showing that some molecules of the amoeba can diffuse away long distances causing cytotoxic effects and even necrosis on hepatic cells of hamster liver without the need of the trophozoite being in close contact with the target cells. They also may promote lytic or proinflammatory effects by inducing the secretion of enzymes or cytokines in other nonparenchymal cells, like PMN leukocytes and endothelial cells. Our results suggest that the accepted mechanisms of cytotoxicity by amoebae are not exclusively restricted to the following sequence: adhesion, phagocytosis, and necrosis.Key words: amoebiasis, Entamoeba histolytica, liver, hamster, immunocytochemistry.Nous avons étudié par immunomicroscopie électronique et immunoperoxydase, les premières étapes de l'interaction in situ entre des trophozoïtes vivants ou fixés d'Entamoeba histolytica et des cellules inflammatoires et du parenchyme hépatique du hamster. Nous avons observé un contact étroit entre les trophozoïtes et les cellules endothéliales et une diffusion de molécules amibiennes des trophozoïtes vers les cellules endothéliales avoisinantes et à distance vers les hépatocytes. Les cellules inflammatoires entourant les amibes ainsi que les cellules parenchymateuses et les hépatocytes restants situés près de la lésion donnaient une réaction positive à une coloration pour la détection des molécules amibiennes. Au niveau de l'ultrastructure des amibes, les molécules se retrouvaient attachées aux membranes et à l'intérieur des vésicules. Ces molécules semblaient apparemment relâchées dans l'espace compris entre le parasite et les cellules endothéliales. Ces cellules endothéliales et les hépatocytes avoisinants et ceux plus éloignés captaient les molécules amibiennes et finalement elles devenaient nécrosées. À l'inverse, lorsque les amibes fixées étaient inoculées, les molécules originant des amibes étaient captées par les cellules endothéliales et les polynuclénaires (PMN), mais celles-ci ne semblaient subir aucun dommage. Notre étude démontre clairement que certaines molécules produites par les amibes peuvent diffuser à longue distance causant ainsi des effets cytotoxiques sur les cellules hétatiques du hamster, allant jusqu'à la nécrose même si les trophozoïtes ne sont pas en contact étroit avec les cellules-cibles. Ces molécules peuvent aussi provoquer des effets lytiques ou proinflammatoires en induisant la sécrétion d'enzymes ou de cytokines chez d'autres cellules non-parenchymateuses comme les leucocytes PMN et les cellules endothéliales. Les résultats de nos expériences suggèrent que les mécanismes de cytotoxicité actuellement reconnus chez les amibes ne seraient pas exclusivement limités à la séquence: adhérence, phagocytose et nécrose.Mots clés : amibiase, Entamoeba histolytica, foie, hamster, immunocytochimie.[Traduit par la Rédaction]


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