Fluorescence Polarization Immunoassays for Metal Ions
- Stopped-flow Fluorescence Polarization Immunoassay. A. Gomez-Hens; M. Aguilar-Caballos // Combinatorial Chemistry & High Throughput Screening;May2003, Vol. 6 Issue 3, p177
A general survey of the analytical application of kinetic methodology in fluorescence polarization immunoassay (FPIA) is presented. Stopped-flow mixing technique (SF) allows the initial rate of the immunochemical reaction between the tracer and the antibody to be obtained, which is used as the...
- Basophil activation test compared to skin prick test and fluorescence enzyme immunoassay for aeroallergen-specific Immunoglobulin-E. Khan, Faisal M.; Ueno-Yamanouchi, Aito; Serushago, Bazir; Bowen, Tom; Lyon, Andrew W.; Lu, Cathy; Storek, Jan // Allergy, Asthma & Clinical Immunology;2012, Vol. 8 Issue 1, p1
Background: Skin prick test (SPT) and fluorescence enzyme immunoassay (FEIA) are widely used for the diagnosis of Immunoglobulin-E (IgE)-mediated allergic disease. Basophil activation test (BAT) could obviate disadvantages of SPT and FEIA. However, it is not known whether BAT gives similar...
- Fluorescence Enhancement of Fluorescein Isothiocyanate- Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma. Ogawa, Takehito; Aoyagi, Satoka; Miyasaka, Takehiro; Sakai, Kiyotaka // Sensors (14248220);2009, Vol. 9 Issue 10, p8271
Fluorescence enhancement of fluorescein isothiocyanate-labeled protein A (FITC-protein A) caused by the binding with immunoglobulin G (IgG) in bovine plasma was studied. FITC-protein A was immobilized onto a glass surface by covalent bonds. An increase in fluorescence intensity was dependent on...
- Using Bead-Based Multiplexing Immunoassays to Explore Cellular Response to Drugs. Houser, Brett // Drug Discovery & Development;May2011, Vol. 14 Issue 5, p23
The article discusses the coupling of immunoglobulins with color-coded beads to measure multiple analytes in multiplex immunoassays.
- Rapid estimation of plasma theophylline concentrations. Cuss, Francis M.; Palmer, James B.; Barnes, Peter J. // British Medical Journal (Clinical Research Edition);8/10/1985, Vol. 291 Issue 6492, p384
The article focuses on a study on the use of a machine which utilizes a technique of solid phase immunochemistry and reflectance photometry to make rapid estimation of plasma theophylline concentrations in the chest clinic. Plasma theophylline concentrations were determined by two operators...
- Nanotechnology breakthrough to improve medical tests. // Advanced Materials & Processes;Jul2012, Vol. 170 Issue 7, p14
The article reports on research into the use of glass and gold nanostructures to amplify the fluorescence signal from biological markers in diagnostic immunoassays, a technology that would improve the sensitivity of the tests by 3 million times.
- Antibody-based Fluorescence Polarization Assay to Screen Combinatorial Libraries for Sweet Taste Compounds. Linthicum, D.S.; Patel, J.; Cairns, N. // Combinatorial Chemistry & High Throughput Screening;Aug2001, Vol. 4 Issue 5, p431
Technological advances in instrumentation, chemical synthesis methods, molecular biology and biochemistry have fueled the recent growth in high throughput screening. Assays are available in a vast range for formats, including fluorescence, luminescence, absorbance, and scintillation detection....
- Simultaneous identification of various antinuclear antibodies using an automated multiparameter line immunoassay system. López-Longo, FJ; Rodríguez-Mahou, M; Escalona-Monge, M; González, Cm; Monteagudo, I; Carreño, L // Lupus;2003, Vol. 12 Issue 8, p623
The objective was to determine the sensitivity and specificity of an automated multiparameter line immunoassay system compared with other techniques for the identification of autoantibodies in rheumatic diseases. We studied sera from 90 patients. Anti-U1RNP, anti-Scl, anti-Ro/SS-A, anti-La/...
- Immunological studies in cattle exposed to polybrominated biphenyls Bazzell, S. J.; Kateley, J. R. // Environmental Health Perspectives;Apr1978, Vol. 23, p75
No abstract available.