TITLE

Spectrofluorimetric and Spectrophotometric Determination of Troxerutin in Pharmaceutical Preparations

AUTHOR(S)
YA HONG CHEN; KAI REN; FENG SHOU TIAN; KE KE ZHU; XIN YAN HU
PUB. DATE
May 2015
SOURCE
Asian Journal of Chemistry;2015, Vol. 27 Issue 5, p1854
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Two new, sensitive and simple spectrofluorimetric and spectrophotometric methods have been developed for the determination of troxerutin in pharmaceutical preparations. Troxerutin inhibits the activity of hemoglobin on the catalyzed reaction of H2O2 with rhodamine B, which is a highly sensitive fluorogenic and chromogenic reagent used in many investigations. The reaction product was measured by spectrofluorimetry at 575 nm after excitation at 550 nm. The percentage inhibition was directly proportional to the concentration over the range 0.04-1.5 µg/mL for the spectrofluorimetric method. The relation between the percentage inhibition of the absorbance at 550 nm and the concentration is rectilinear over the range 0.2-30 µg/mL. This method was applied successfully to the determination of troxerutin in pharmaceutical dosage form.
ACCESSION #
103336551

 

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