TITLE

MICROPROPAGATION OF JARTORPHA CURCAS L. THROUGH SHOOT TIP EXPLANTS USING DIFFERENT CONCENTRATIONS OF PHYTOHORMONES

AUTHOR(S)
Imtiaz, M.; Khattak, A. M.; Ara, N.; Iqbal, A.; Rahman, H. U.
PUB. DATE
January 2014
SOURCE
JAPS: Journal of Animal & Plant Sciences;2014, Vol. 24 Issue 1, p229
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A study was conducted to evaluate the effect of phytohormone BAP alone and in combination with IBA or NAA for invitro propagation of Jatropha curcas shoot tip explants. MS medium supplemented with various concentrations of phytohormones significantly affected shoot induction. Maximum (93.33%) shoot induction occurred when MS medium was supplemented with 8.88 μM BAP or 13.32 and 4.92 μM of BAP and IBA respectively. Similarly, maximum shoot buds per explant (6.7) and shoot length (4.5 cm) were obtained when 13.32 μM BAP and 4.92μM IBA was added to the MS medium. Shoots produced were re-cultured to MS, ½ MS media and ½ MS medium supplemented with different concentrations of IBA and were studied for rooting, where the response was highly significant. IBA supplemented to ½ MS medium at 14.76μM gave maximum (4.93) roots per culture.
ACCESSION #
103210638

 

Related Articles

  • MULTIPLICACIÓN IN VITRO DE BROTES DE TRES VARIEDADES DE CALLAS (Zantedeschia sp.) EMPLEANDO SISTEMA DE INMERSIÓN TEMPORAL. Sánchez, Javier; e Iris Capote, Marcos Daquinta // Ciencia y Tecnología (1390-4051);2010, Vol. 3 Issue 1, p1 

    Callas (Zantedeschia spp.) belonging to Araceae family are ornamental plants with high commercial demand as plants in pot and cut flower. Traditional propagation techniques are not able to satisfy the fast introduction of new hybrids to the market. Thus, in vitro culture techniques are a useful...

  • Lack of Cytosolic Glutamine Synthetase1;2 Activity Reduces Nitrogen-Dependent Biosynthesis of Cytokinin Required for Axillary Bud Outgrowth in Rice Seedlings. Miwa Ohashi; Keiki Ishiyama; Soichi Kojima; Mikiko Kojima; Hitoshi Sakakibara; Tomoyuki Yamaya; Toshihiko Hayakawa // Plant & Cell Physiology;Apr2017, Vol. 58 Issue 4, p679 

    A mutation abolishing cytosolic glutamine synthetase1;2 (GS1;2) activity impairs assimilation of ammonium into glutamine in both roots and basal portions of shoots, and severely decreases axillary bud outgrowth (tillering) in mutant rice seedlings. Although the gs1;2 mutant phenotype is...

  • In vitro regeneration of plantlets from mature embryos of Pinus ayacahuite. Saborio, Francisco; Dvorak, William S.; Donahue, Jeffrey K.; Thorpe, Trevor A. // Tree Physiology;Dec1997, Vol. 17 Issue 12, p787 

    A plantlet regeneration protocol was developed for Pinus ayacahuite var. ayacahuite (Ehrenb.). Embryos from mature seeds from ten provenances were cultured in a 16-h photoperiod for 3 days on a medium containing 30 mM sucrose and 0.7% agar. Cotyledons from these embryos were subcultured onto MCM...

  • Micropropagation of Salvadora persica-a tree of arid horticulture and forestry. Phulwaria, M.; Ram, Kheta; Gahlot, Priyanka; Shekhawat, N. // New Forests;Nov2011, Vol. 42 Issue 3, p317 

    A micropropagation method for Jaal ( Salvadora persica)-a tree of arid horticulture and forestry has been developed. Nodal segments of fresh shoot sprouts originated from axillary buds obtained from a plant around 35-40 years old lopped plant were used as explants for establishment of in vitro...

  • In Vitro Regeneration of Phyllanthus amarus Schum. and Thonn.: An Important Medicinal Plant. Sen, A.; Sharma, M. M.; Grover, D.; Batra, A. // Our Nature;2009, Vol. 7 Issue 1, p110 

    An efficient in vitro plant regeneration protocol was developed for the medicinally potent plant species Phyllanthus amarus Schum. and Thonn. (Euphorbiaceae) using nodal segment as explant. Maximum multiplication of shoots (15.275±0.96) was achieved on Murashige and Skoog's medium...

  • High Frequency Shoot Multiplication and Callus Regeneration of Turmeric. Roopadarshini, V. // International Journal of Biotechnology & Biochemistry;Oct2010, Vol. 6 Issue 5, p723 

    An efficient protocol for shoot multiplication and callus regeneration has been standardized in an important spice plant, Curcuma longa L. (turmeric) variety Suguna. LSBM fortified with BAP (3.0 mgl-1) was found to be the suitable medium for initiation and multiplication of shoots (1:36) from...

  • Rapid clonal propagation of an endangered medicinal plant Plumbago zeylanica Linn. Dohare, Bharti; Jain, Kirti; jain, Bharti; Khare, Swati // International Journal of Pharmacy & Life Sciences;Aug2012, Vol. 3 Issue 8, p1883 

    The Present investigation has given a method for in vitro micropropagation of a valued endangered medicinal plant Plumbago zeylanica Linn. (Plumbaginaceae) called chitrak. In this study we used nodal part of P .zeylanica as explant. Explants were cultured on MS media (Murashige and Skoog)...

  • Increased root and shoot production during micropropagation of cherry and apple rootstocks: effect of subculture frequency. Grant, N. J.; Hammatt, N. // Tree Physiology;Nov1999, Vol. 19 Issue 13, p899 

    Shoot cultures of apple rootstock cv. M9 and cherry rootstock cv. F12/1 were established and then divided into several sublines that were subcultured at 28–42-day intervals. Consequently, similarly aged cultures received various numbers of subcultures. Cultures kept at 24 °C showed an...

  • Micropropagation of Nyctanthes arbor-tristis. Raja, H. David; Arockiasamy, D. I. // Journal of Tropical Medicinal Plants;Dec2008, Vol. 9 Issue 2, p400 

    An efficient protocol for rapid mass in vitro propagation of Nyctanthes arbor-tristis L. nodal explants is outlined. Maximum number of shoots was obtained in 2.5mg/l BAP + 5% CW medium. High frequency of root proliferation from the nodal explant was achieved in 2mg/l of IBA medium. The rooted...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics