TITLE

PAX6 does not regulate Nfia and Nfib expression during neocortical development

AUTHOR(S)
Bunt, Jens; Lim, Jonathan W. C.; Zhao, Lu; Mason, Sharon; Richards, Linda J.
PUB. DATE
May 2015
SOURCE
Scientific Reports;5/29/2015, p10668
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The Nuclear factor I (NFI) family of transcription factors regulates proliferation and differentiation throughout the developing central nervous system. In the developing telencephalon of humans and mice, reduced Nfi expression is associated with agenesis of the corpus callosum and other neurodevelopmental defects. Currently, little is known about how Nfi expression is regulated during early telencephalic development. PAX6, a transcription factor important for telencephalic development, has been proposed as an upstream regulator of Nfi expression in the neocortex. Here we demonstrate that, in the developing neocortex of mice, NFIA and NFIB are endogenously expressed in gradients with high caudo-medial to low rostro-lateral expression and are most highly expressed in the cortical plate. We found that this expression pattern deviates from that of PAX6, suggesting that PAX6 does not drive Nfi expression. This is supported by in vitro reporter assays showing that PAX6 over-expression does not regulate Nfi promoter activity. Similarly, we also found that in the Pax6 Small Eye mutant, no changes in Nfi mRNA or protein expression are observed in the neocortical ventricular zone where PAX6 and the NFIs are expressed. Together these data demonstrate that in mice, PAX6 is not a transcriptional activator of Nfi expression during neocortical development.
ACCESSION #
102953953

 

Related Articles

  • Preservation of positional identity in fetus-derived neural stem (NS) cells from different mouse central nervous system compartments. Onorati, Marco; Binetti, Maurizio; Conti, Luciano; Camnasio, Stefano; Calabrese, Giovanna; Albieri, Ilaria; Febo, Francesca; Toselli, Mauro; Biella, Gerardo; Martynoga, Ben; Guillemot, Francois; Consalez, G.; Cattaneo, Elena // Cellular & Molecular Life Sciences;May2011, Vol. 68 Issue 10, p1769 

    Neural stem (NS) cells are a self-renewing population of symmetrically dividing multipotent radial glia-like stem cells, characterized by homogeneous expansion in monolayer. Here we report that fetal NS cells isolated from different regions of the developing mouse nervous system behave in a...

  • cerebrum.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p411 

    A definition of the term "cerebrum" is presented. It refers to the largest part of the brain consisting of two hemispheres which are united by the corpus callosum, the anterior and posterior hippocampal commissures, and separated by a deep longitudinal fissure. The embryology, anatomy, and...

  • AGENEZIE DE CORP CALOS - PREZENTARE RADIOIMAGISTICÄ‚. DINULESCU, DANIELA; CHEREGI, C.; SABĂU, MONICA; POP, O.; RAHOTĂ, DANIELA // Clujul Medical;Sep2011 Supplement, p136 

    Introduction Agenesis of corpus callosum is a rare congenital disease (present at birth), characterized by partial or total absence ( due to lack of training - agenesis) of a region of the brain that connects the two cerebral hemispheres. Corpus callosum is generally made available from the...

  • Characteristics of differentiated CD8 and CD4 T cells present in the human brain. Smolders, Joost; Remmerswaal, Ester; Schuurman, Karianne; Melief, Jeroen; Eden, Corbert; Lier, René; Huitinga, Inge; Hamann, Jörg // Acta Neuropathologica;Oct2013, Vol. 126 Issue 4, p525 

    Immune surveillance of the central nervous system (CNS) by T cells is important to keep CNS-trophic viruses in a latent state, yet our knowledge of the characteristics of CNS-populating T cells is incomplete. We performed a comprehensive, multi-color flow-cytometric analysis of isolated T cells...

  • Koro secondary to a tumour of the corpus callosum. Durst, Rimona; Rosca-Revaudengo, Paola; Durst, R; Rosca-Rebaudengo, P // British Journal of Psychiatry;Aug88, Vol. 153, p251 

    The concurrent appearance of the koro syndrome and pathology of the central nervous system is quite rare. A case of a patient with a diagnosed tumour in the genu of the corpus callosum, who displayed typical koro symptoms, is presented. A short series of electroconvulsive shock treatments...

  • The Ciliogenic Transcription Factor RFX3 Regulates Early Midline Distribution of Guidepost Neurons Required for Corpus Callosum Development. Benadiba, Carine; Magnani, Dario; Niquille, Mathieu; Morlé, Laurette; Valloton, Delphine; Nawabi, Homaira; Ait-Lounis, Aouatef; Otsmane, Belkacem; Reith, Walter; Theil, Thomas; Hornung, Jean-Pierre; Lebrand, Cécile; Durand, Bénédicte // PLoS Genetics;Mar2012, Vol. 8 Issue 3, Special section p1 

    The corpus callosum (CC) is the major commissure that bridges the cerebral hemispheres. Agenesis of the CC is associated with human ciliopathies, but the origin of this default is unclear. Regulatory Factor X3 (RFX3) is a transcription factor involved in the control of ciliogenesis, and...

  • Role of SOX family of transcription factors in central nervous system tumors. de la Rocha, Arlet M. Acanda; Sampron, Nicolas; Alonso, Marta M.; Matheu, Ander // American Journal of Cancer Research;2014, Vol. 4 Issue 4, p312 

    SOX genes are developmental regulators with functions in the instruction of cell fate and maintenance of progenitor's identity during embryogenesis. They play additional roles during tissue homeostasis and regeneration in adults particularly in the Central Nervous System (CNS). In the last years...

  • Generation of mice encoding a conditional allele of Nkx2.2. Mastracci, Teresa; Lin, Chyuan-Sheng; Sussel, Lori // Transgenic Research;Oct2013, Vol. 22 Issue 5, p965 

    Nkx2.2 is a homeobox transcription factor that is expressed in the pancreas, intestine and central nervous system (CNS) during embryogenesis and in the adult. In mice, global deletion of Nkx2.2 results in cell mis-specification in each of the tissues where it is expressed, and the null mice die...

  • Comparative genomics using teleost fish helps to systematically identify target gene bodies of functionally defined human enhancers. Parveen, Nazia; Masood, Ayesha; Iftikhar, Nouman; Minhas, Bushra F.; Minhas, Rashid; Nawaz, Uzma; Abbasi, Amir Ali // BMC Genomics;2013, Vol. 14 Issue 1, p1 

    Background: Human genome is enriched with thousands of conserved non-coding elements (CNEs). Recently, a medium throughput strategy was employed to analyze the ability of human CNEs to drive tissue specific expression during mouse embryogenesis. These data led to the establishment of publicly...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics