TITLE

Addition of L-dopa to HBSS in enhancing the maintenance of cell viability of Periodontal Ligament (PDL) cells: An In-Vitro Study

AUTHOR(S)
EBENEZAR, A. V. RAJESH; MAHENDRAN, T.; PRIYA, JANANI
PUB. DATE
October 2014
SOURCE
Journal of Clinical & Diagnostic Research;Oct2014, Vol. 8 Issue 10, p79
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Aim: To investigate the viability of PDL cells in an avulsed tooth using a storage medium with and without L-dopa. Materials and Methods: Twenty freshly extracted non-impacted human teeth with closed apices and no caries beyond the CEJ were obtained for this study. The teeth were then randomly divided into 1 of the 4 experimental storage solution groups, namely group 1: deionized water; group 2: deionized water with L-dopa (1μg/μl); group 3: HBSS; and group 4: HBSS with L-dopa (1μig/μl). After storing the teeth in different test solutions for 45 minutes collagenase assay was performed and the number of viable PDL cells were counted under a light microscope using a hemocytometer at 20X magnification. Results: The mean number of viable PDL cells was highest in group 4, followed by group 3 and group 2Group 1 shows the least number of viable PDL cells. Conclusion: The synergistic effect of L-dopa and HBSS paves way for its usage as an effective storage medium for an avulsed tooth
ACCESSION #
101633088

 

Related Articles

  • CLINICO-MORPHOLOGICAL RESEARCH OF BIO-OSS ® DURING BONE-PLASTIC OPERATIONS. SIDELNIKOV, Pavel // International Journal of Medical Dentistry;Jan-Mar2015, Vol. 19 Issue 1, p23 

    Aim: To study the clinical and morphological characteristics of Bio-Oss ® and Bio-Gate ® materials during bone-plastic operations, especially bone regeneration after surgical interventiond. Materials and method: The pathomorphological study was performed with the intravital biopsy material...

  • Evaluation and comparison of efficacy of three different storage media, coconut water, propolis, and oral rehydration solution, in maintaining the viability of periodontal ligament cells. Sanghavi, Tulsi; Shah, Nimisha; Parekh, Vaishali; Singbal, Kiran // Journal of Conservative Dentistry;Jan/Feb2013, Vol. 16 Issue 1, p71 

    Background: Two of the most critical factors affecting the prognosis of an avulsed tooth after replantation are extra oral dry time and the storage medium in which the tooth is placed before treatment is rendered. However, the ability of a storage/transport medium to support cell viability can...

  • Disease-associated extracellular matrix suppresses osteoblastic differentiation of human periodontal ligament cells via MMP-1. Joseph, Jeena; Kapila, Yvonne L.; Hayami, Takayuki; Kapila, Sunil // Calcified Tissue International;Feb2010, Vol. 86 Issue 2, p154 

    Fibronectin (FN) fragments found in chronic inflammatory diseases, including periodontal disease and arthritis, may contribute to tissue destruction in part via induction of matrix metalloproteinases (MMPs). We previously showed that the 120-kDa FN fragment containing the central cell binding...

  • Periodontal Ligament Cells are Chemotactic to Fibroblast Collagenase. Terranova, V. P.; Nishimura, F. // Journal of Dental Research;Apr1996, Vol. 75 Issue 4, p993 

    Periodontal ligament (PDL) cell motility and the passage of PDL cells along a root surface are important components of tissue remodeling during periodontal regeneration. Proteolytic enzymes, including fibroblast collagenase, have been demonstrated to play an important role in tissue remodeling....

  • Presence of Collagenase from Clostridium histolyticum in Gingival Sulcal Debris of a Primitive Population. ALFANO, MICHAEL C.; MORHART, ROBERT E.; METCALF, GUY; DRUMMOND, JAMES F. // Journal of Dental Research;Jan1974, Vol. 53 Issue 1, p142 

    The article discusses research which examines the presence of Clostridium histolyticum in gingival sulcal debris. Details are provided about the production of extracellular collagenase by Clostridium histolyticum. The role of collagenase in the early breakdown of periodontal tissues in primitive...

  • Gingival Tissue and Crevicular Fluid Co-operation in Adult Periodontitis. Beklen, A.; Tüter, G.; Sorsa, T.; Hanemaaijer, R.; Virtanen, I.; Tervahartiala, T.; Konttinen, Y. T. // Journal of Dental Research;Jan2006, Vol. 85 Issue 1, p59 

    Activated matrix metalloproteinase-3 (MMP-3) can contribute to periodontal ligament destruction in adult periodontitis. Since MMP-3 has been reported to activate proMMP-8 and -9, it was speculated that gingival tissue fibroblast-derived MMP-3 might, in periodontitis, be responsible for...

  • Comparison of Periodontal Ligament Stem Cells Isolated from the Periodontium of Healthy Teeth and Periodontitis-Affected Teeth. Soheilifar, Sara; Amiri, Iraj; Bidgoli, Mohsen; Hedayatipanah, Morad // Journal of Dentistry of Tehran University of Medical Sciences;Aug2016, Vol. 13 Issue 4, p271 

    Objectives: Stem cell (SC) therapy is a promising technique for tissue regeneration. This study aimed to compare the viability and proliferation ability of periodontal ligament stem cells (PDLSCs) isolated from the periodontium of healthy and periodontitis-affected teeth to obtain an autologous,...

  • Piceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative Stress. Costa, Flávia Póvoa da; Puty, Bruna; Nogueira, Lygia S.; Mitre, Geovanni Pereira; Santos, Sávio Monteiro dos; Teixeira, Bruno José Brito; Kataoka, Maria Sueli da Silva; Martins, Manoela Domingues; Barboza, Carlos Augusto Galvão; Monteiro, Marta Chagas; Rogez, Hervé; Oliveira, Edivaldo Herculano Corrêa de; Lima, Rafael Rodrigues // Antioxidants;Jan2020, Vol. 9 Issue 1, p16 

    Piceatannol is a resveratrol metabolite that is considered a potent antioxidant and cytoprotector because of its high capacity to chelate/sequester reactive oxygen species. In pathogenesis of periodontal diseases, the imbalance of reactive oxygen species is closely related to the disorder in the...

  • The Effect of Propolis as A Biological Storage Media on Periodontal Ligament Cell Survival in An Avulsed Tooth: An In Vitro Study. Ahangari, Zohreh; Alborzi, Samiye; Yadegari, Zahra; Dehghani, Fatemeh; Ahangari, Leila; Naseri, Mandana // Cell Journal (Yakhteh);Autumn2013, Vol. 15 Issue 3, p244 

    Objective: Both the length of extra-alveolar time and type of storage media are significant factors that can affect the long-term prognosis of replanted teeth. This study aims to compare propolis 50%, propolis 10%, Hank's balanced salt solution (HBSS), milk and egg white on periodontal ligament...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics