Cafeteria diet overfeeding in young male rats impairs the adaptive response to fed/fasted conditions and increases adiposity independent of body weight

Castro, H; Pomar, C A; Picó, C; Sánchez, J; Palou, A
March 2015
International Journal of Obesity;Mar2015, Vol. 39 Issue 3, p430
Academic Journal
Objective:We analyzed the effects of a short exposure to a cafeteria diet during early infancy in rats on their metabolic response to fed/fasting conditions in key tissues involved in energy homeostasis.Methods:Ten-day-old male pups were fed a control or a cafeteria diet for 12 days and then killed under ad libitum feeding conditions or 12 h fasting. The expression of key genes related to energy metabolism in liver, retroperitoneal white adipose tissue (WAT) and hypothalamus were analyzed.Results:Despite no differences in body weight, cafeteria-fed animals had almost double the fat mass of control rats. They also showed higher food intake, higher leptinemia and altered hypothalamic expression of Neuropetide Y, suggesting a dysfunction in the control of food intake. Unlike controls, cafeteria-fed animals did not decrease WAT expression of Pparg, sterol regulatory element binding transcription factor 1 or Cidea under fasting conditions, and displayed lower Pnpla2 expression than controls. In liver, compared with controls, cafeteria animals presented: (i) lower expression of genes related with fatty acid uptake and lipogenesis under ad libitum-fed conditions; (ii) higher expression of fatty acid oxidation-related genes and glucokinase under fasting conditions; (iii) greater expression of leptin and insulin receptors; and higher protein levels of insulin receptor and the pAMPK/AMPK ratio.Conclusion:A short period of exposure to a cafeteria diet in early infancy in rat pups is enough to disturb the metabolic response to fed/fasting conditions in key tissues involved in energy homeostasis, particularly in WAT, and hence induces an exacerbated body fat accumulation and increased metabolic risk, with no apparent effects on body weight.


Related Articles

  • Proteomic Identification of Novel Differentiation Plasma Protein Markers in Hypobaric Hypoxia-Induced Rat Model. Ahmad, Yasmin; Sharma, Narendra K.; Ahmad, Mohammad Faiz; Sharma, Manish; Garg, Iti; Bhargava, Kalpana // PLoS ONE;May2014, Vol. 9 Issue 5, p1 

    Background: Hypobaric hypoxia causes complex changes in the expression of genes, including stress related genes and corresponding proteins that are necessary to maintain homeostasis. Whereas most prior studies focused on single proteins, newer methods allowing the simultaneous study of many...

  • In Vivo Initiation of Clock Gene Expression Rhythmicity in Fetal Rat Suprachiasmatic Nuclei. Houdek, Pavel; Sumová, Alena // PLoS ONE;Sep2014, Vol. 9 Issue 9, p1 

    The mammalian suprachiasmatic nuclei (SCN) and their intrinsic rhythmicity develop gradually during ontogenesis. In the rat, the SCN forms between embryonic day (E) 14 and E17, with gestation terminating at E21–22. Overt SCN rhythmicity is already present in the late embryonic stage. The...

  • Regulation of glucose homeostasis using radiogenetics and magnetogenetics in mice. Leibiger, Ingo B; Berggren, Per-Olof // Nature Medicine;Jan2015, Vol. 21 Issue 1, p14 

    The authors focus on a study published in journal "Nature Medicine" related to noninvasively control of gene expression by the application of radiogenetics and magnetic field in mice. Topics discussed include use of nanoparticle to control transgene expression and glucose homeostasis in a...

  • Niacin in Pharmacological Doses Alters MicroRNA Expression in Skeletal Muscle of Obese Zucker Rats. Couturier, Aline; Keller, Janine; Most, Erika; Ringseis, Robert; Eder, Klaus // PLoS ONE;May2014, Vol. 9 Issue 5, p1 

    Administration of pharmacological niacin doses was recently reported to have pronounced effects on skeletal muscle gene expression and phenotype in obese Zucker rats, with the molecular mechanisms underlying the alteration of gene expression being completely unknown. Since miRNAs have been shown...

  • Changes in Different Insulin Sensitive Tissues Gene Expression of Cat Fed on High-fat Diet. Li, Gebin; Lee, Peter; Yuki Okada; Ichiro Yamamoto; Toshiro Arai; Nobuko Mori // Asian Journal of Animal & Veterinary Advances;2014, Vol. 9 Issue 5, p270 

    The objective of this study was to determine whether Peripheral Blood Leukocytes (PBL) are sensitive enough to detect early signs of diet induced obesity related changes occurring in insulin sensitive tissues, such as abdominal omental and subcutaneous adipose, liver and skeletal muscle, by...

  • Remote regulation of glucose homeostasis in mice using genetically encoded nanoparticles. Stanley, Sarah A; Sauer, Jeremy; Kane, Ravi S; Dordick, Jonathan S; Friedman, Jeffrey M // Nature Medicine;Jan2015, Vol. 21 Issue 1, p92 

    Means for temporally regulating gene expression and cellular activity are invaluable for elucidating underlying physiological processes and would have therapeutic implications. Here we report the development of a genetically encoded system for remote regulation of gene expression by...

  • Early Postnatal Nutrition Determines Adult Physical Activity and Energy Expenditure in Female Mice. Ge Li; Kohorst, John J.; Wenjuan Zhang; Laritsky, Eleonora; Kunde-Ramamoorthy, Govindarajan; Baker, Maria S.; Fiorotto, Marta L.; Waterland, Robert A. // Diabetes;Aug2013, Vol. 62 Issue 8, p2773 

    Decades of research in rodent models has shown that early postnatal overnutrition induces excess adiposity and other components of metabolic syndrome that persist into adulthood. The specific biologic mechanisms explaining the persistence of these effects, however, remain unknown. On postnatal...

  • Single Rapamycin Administration Induces Prolonged Downward Shift in Defended Body Weight in Rats. Hebert, Mark; Licursi, Maria; Jensen, Brittany; Baker, Ashley; Milway, Steve; Malsbury, Charles; Grant, Virginia L.; Adamec, Robert; Hirasawa, Michiru; Blundell, Jacqueline // PLoS ONE;May2014, Vol. 9 Issue 5, p1 

    Manipulation of body weight set point may be an effective weight loss and maintenance strategy as the homeostatic mechanism governing energy balance remains intact even in obese conditions and counters the effort to lose weight. However, how the set point is determined is not well understood. We...

  • Chronic activation of cannabinoid receptors in vitro does not compromise mouse islet function. VILCHES-FLORES, Alonso; HAUGE-EVANS, Astrid C.; JONES, Peter M.; PERSAUD, Shanta J. // Clinical Science;2013, Vol. 124 Issue 7, p467 

    We have demonstrated previously that mouse and human islets express ECS (endocannabinoid system) elements, and that short-term activation of islet cannabinoid CB1r and CB2r (cannabinoid type 1 and 2 receptors respectively) stimulates insulin secretion in vitro. There is evidence that the ECS is...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics