TITLE

Benchtop fluorometry of phycocyanin as a rapid approach for estimating cyanobacterial biovolume

AUTHOR(S)
Kasinak, Jo-Marie E.; Holt, Brittany M.; Chislock, Michael F.; Wilson, Alan E.
PUB. DATE
January 2015
SOURCE
Journal of Plankton Research;Jan2015, Vol. 37 Issue 1, p248
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Cyanobacteria are the primary taxa responsible for freshwater harmful algal blooms (HABs), with several genera capable of producing potent intracellular toxins and off-flavor compounds. There is considerable growing interest in methods to rapidly quantify cyanobacteria in water samples. Past studies have demonstrated poor correlations between phycocyanin in vivo fluorescence and cyanobacterial cell densities. We conducted a series of laboratory experiments aimed at refining a protocol that uses benchtop fluorometry to measure the cyanobacterial pigment, phycocyanin, to accurately estimate cyanobacterial biovolume. In our study, we found strong correlations between phycocyanin concentration and cyanobacterial biovolume (but not for cell densities) both within and across ponds, which varied widely in productivity and algal diversity. Thus, benchtop fluorometry of phycocyanin is a viable method for water resource managers to quickly estimate cyanobacterial biovolume.
ACCESSION #
101035656

 

Related Articles

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics