Preliminary findings of quorum signal molecules in clinically stable lung allograft recipients

Ward, C.; Cámara, M.; Forrest, I.; Rutherford, R.; Pritchard, G.; Daykin, M.; Hardman, A.; de Soyza, A.; Fisher, A.J.; Williams, P.; Corris, P.A.
May 2003
Thorax;May2003, Vol. 58 Issue 5, p444
Academic Journal
Background: Infection with bacteria such as Pseudomonas is common in lung allograft recipients, particularly during chronic rejection. Analysis of sputum samples from patients with cystic fibrosis infected with Pseudomonas aeruginosa or Burkholderia cepacia has indicated the presence of bacterial N-acylhomoserine lactones (AHLs) quorum sensing signalling molecules. AHLs not only control the expression of bacterial virulence genes but are also involved in stimulating the maturation of antibiotic resistant biofilms and host chemokine release. It was hypothesised that AHLs may be detected even in clinically stable lung transplant recipients free of clinical infection or rejection. Methods: Three 60 ml samples of bronchoalveolar lavage (BAL) fluid were taken from nine stable lung transplant recipients 3-12 months after transplantation. Detection of AHLs was carried out on dichloromethane extracted supernatants using the bioluminescence based AHL reporter plasmid pSB1075. This responds to the presence of AHLs with long acyl chains (C10-C14), generating light. Synthetic AHLs were included as positive controls. Results: Five of the nine BAL fluid supernatants exhibited AHL activity, suggesting the presence of AHLs with long N-acyl chains. There was no correlation between the levels of AHLs detected or their absence and BAL fluid microbiology or diagnosis before transplantation. Conclusions: This is the first evidence for the presence of AHL quorum sensing signals in human lung allograft recipients, even in subjects with no rejection or apparent infection. Further longitudinal follow up of these preliminary findings is required to elucidate potential links with infection, rejection, and allograft deterioration.


Related Articles

  • Non Mycobacterial Virulence Genes in the Genome of the Emerging Pathogen Mycobacterium abscessus. Ripoll, Fabienne; Pasek, Sophie; Schenowitz, Chantal; Dossat, Carole; Barbe, Valérie; Rottman, Martin; Macheras, Edouard; Heym, Beate; Herrmann, Jean-Louis; Daffé, Mamadou; Brosch, Roland; Risler, Jean-Loup; Gaillard, Jean-Louis // PLoS ONE;2009, Vol. 4 Issue 6, p1 

    Mycobacterium abscessus is an emerging rapidly growing mycobacterium (RGM) causing a pseudotuberculous lung disease to which patients with cystic fibrosis (CF) are particularly susceptible. We report here its complete genome sequence. The genome of M. abscessus (CIP 104536T) consists of a...

  • Decolorization does not always mean detoxification: case study of a newly isolated Pseudomonas peli for decolorization of textile wastewater. Dellai, Afef; Dridi, Dorra; Lemorvan, Valerie; Robert, Jacques; Cherif, Ameur; Mosrati, Ridha; Mansour, Hedi // Environmental Science & Pollution Research;Aug2013, Vol. 20 Issue 8, p5790 

    The textile industry is a favor to the Tunisian economy by offering several job positions. However, it's not environmentally friendly. In fact, textile industries discharge high volumes of wastewater which contain several toxic pollutants such as dyes, fixator, and whiteness. In our study,...

  • The debrisoquine metabolic phenotype and DNA-based assays: implications of misclassification for the association of lung cancer and the debrisoquine metabolic phenotype Hoover, R.; Weston, A.; Sugimura, H.; Shields, P. G.; Caporaso, N. E.; Harris, C. C.; Landi, M. T.; Shaw, G. L.; Tucker, M. A. // Environmental Health Perspectives;Nov1992, Vol. 98, p101 

    No abstract available.

  • Bird in hand...may be deadly. Segell, Michael // Cosmopolitan;Feb1993, Vol. 214 Issue 2, p50 

    Reports that keeping a pet bird at home significantly increases your chances of developing lung cancer, according to findings recently published in the `British Medical Journal.' Results of a study; Possible cause; Advice.

  • Lung cancer: A puzzling disease. E.B. // Medical Update;Oct95, Vol. 19 Issue 4, p3 

    Reports on different types of lung cancer. Increase in the incidence of non-smoking related lung cancer; Importance of determining the specific cell type of cancer; Treatment as consisting of surgery, radiation, chemotherapy or combinations; Contact information.

  • Stage IIIA category of non-small-cell lung cancer: A new proposal. Green, Mark R.; Lilenbaum, Rogerio C. // JNCI: Journal of the National Cancer Institute;4/20/94, Vol. 86 Issue 8, p586 

    Opinion. Focuses on a proposed International Staging System (ISS) for lung cancer. Categories; Redefinition of stage III disease; Prognostic differences between patients; Recommendations.

  • Residential radon exposure and lung cancer: evidence of an inverse association in Washington state Gerald, Kenneth B.; Neuberger, John S.; Frost, Floyd J. // Journal of Environmental Health;Nov/Dec1992, Vol. 55 Issue 3, p23 

    No abstract available.

  • Uncool, unsmooth, unhealthy. Farrell, Liam // BMJ: British Medical Journal (International Edition);8/3/96, Vol. 313 Issue 7052, p306 

    Focuses on the incidence of cancer in Great Britain. Symptoms of lung cancer; Diagnosis and management of cancer.

  • Tobacco wars.  // Patient Care;6/15/1997, Vol. 31 Issue 11, p13 

    Discusses trends surrounding lung cancer such as the steep increase in deaths from the disease in women based on the national videoconference `Lung cancer: Taking control,' held at the National Institutes of Health in Bethesda, Maryland. Discussion about the genetic markers of lung cancer, tests...


Read the Article


Sign out of this library

Other Topics