Assessment of sperm parameters and chromatin status in rat experimental varicocele

Afiyani, A. Atrian; Azadi, L.; Deemeh, M.; Tavalaee, M.; Nasr-Esfahani, M.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p121
Academic Journal
Introduction: Varicocele as a common cause of male infertility is introduced and impairs thermoregulation of testis by disrupting the countercurrent heat exchange mechanism in the pampiniform venous plexus and affect spermatogenesis and sperm function. In addition, it prones sperm to chromatin damage and morphological alternation. Therefore, we aimed to evaluate the effect of varicocele induction on semen parameters and chromatin status in a rat's model. Materials and Methods: 23 Wistar male rats were divided into three groups including left varicocele, control-sham and control groups. Left varicocele was induced by surgery. Two months after the surgery, all the rats were anaesthetized and sperm from bilateral caudal segment of epididymis were extracted. Sperm concentration was determined with Mackler chamber. Sperm motility was assessed by light microscopy using pre-warmed slide. Sperm morphology was assessed following Eosin/Nigrosin staining.DNA fragmentation and protamine deflciency were analyzed byacridine orange and chromomycin A3 staining, respectively. Results: In the varicocele group, sperm concentration, percentage of sperm motility and normal morphology were signiflcantly reduced in the left epididymis compared to the right. Also percentage of these parameters was signiflcantly reduced in the varicocele group than control-sham and control groups. In the varicocele group, percentage of sperm DNA fragmentation and protamine deficiency were signiflcantly higher in left compared to right. Also percentage of these parameters was signiflcantly higher in the varicocele group than control-sham and control groups. Conclusion: Induction of varicocele has negative effects on spermatogenesis and following it, on sperm quality, sperm production and destruction of sperm chromatin structure.


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