KLC3-gene expression comparison between fertile and infertile individuals

Dastjerdy, P. Kargar; Azadi, L.; Deemeh, M.; Tavalaee, M.; Salehi, M.; Falahati, M.; Esfahani, M. Nasr
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p120
Academic Journal
Introduction: KLC3 protein as a member of the kinesin light-chain protein family plays an important role in spermatogenesis, by forming a mitochondrial sheath in the mid-piece of the sperm tail. The expression of this gene in mutated mice was lower than in the wild-type mice, which leads to the reduction of sperm parameters quality. This study, for the first time, aimed to compare the expression of the KLC3 gene between fertile and infertile individuals. Materials and Methods: The current study is a case-control. Semen samples were collected from 19 fertile and 57 infertile individuals. The infertile group consisted of men who had abnormal sperm parameters according to world health organization (WHO) criteria, and the fertile group was selected from embryo-donor volunteers.Sperm parameters using computer assisted sperm analysis (CASA) and the quantitative KLC3-gene expression using the real-time PCR method were measured. Results were analyzed by SPSS (version 18). Results: Our results revealed that the KLC3-gene expression in fertile individuals significantly higher than those infertile (p=0.036). The correlation efficiency between quantitative KLC3-gene expression and sperm parameters show a significant correlation between sperm concentration and the KLC3-gene expression (r=0.24). Conclusion: We concluded that absence of KLC3-gene is associated with abnormal spermatogenesis and defects in sperm motility. Presence of this gene is indicator of correct testicular function in the spermatogenesis stage.


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