TITLE

Comparison of human sperm capacitation status using the measured hyper-activation motility pattern between the DGC and DGC-PVP procedures

AUTHOR(S)
Boldaji, B. Torki; Azadi, L.; Tavalaee, M.; Bahadorani, M.; Nasr-Esfahani, M.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p119
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: Coinciding Polyvinyl-pyrrolidone (PVP) high viscosity, clinical center use from PVP for decreasing motility of sperm during ICSI (Intracytoplasmic sperm injection) technique. In animal model, it has been shown that PVP can affect sperm capacitation, Capacitation is a very complex process. One of the visible markers for capacitation is hyperactivation motility. Therefore, the aim of this study was to study of human sperm capacitation status using the measured hyper-activation motility pattem and compare it between two sperm selection procedures: the density gradient centrifugation (DGC) and DGC-PVP. Materials and Methods: Semen samples (n=10) were collected from infertile individuals and semen parameters were evaluated according to WHO criteria. Each semen sample divided into three portions. A portion of the sample was fresh semen sample and second portion was used for DGC procedure. The third portion was considered as DGC-PVP procedure. Kinematic characteristics of sperm in all groups were assessed using computer assisted sperm analysis (CASA), Results were analyzed by SPSS software version 18:5, Results: The results of this study showed that percentage of sperm hyper-active in DGC-PVP (2.01%) and semen (5.8%) significantly less than DGC (21.13%), Conclusion: High percentage of sperm hyper-activated in DGC group reflected that of sperm was more underwent capacitation and such sperm cannot enter DNA damage and apoptosis process. Therefore, selection of sperm from DGC procedure is useful for successful fertilization and pregnancy. PVP is useful for immotility of sperm after DGC and the better selection of sperm based on morphology during ICSI procedure.
ACCESSION #
96841785

 

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