Evaluation of cryopreserved human spermatozoa parameters after incubating with Iranian traditional herb (Calligonum) extract

Ekrami, H.; Movahedin, M.; Mazaheri, Z.; Dizaji, M. Mokhtari
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p118
Academic Journal
Introduction: Nowadays, freezing process is a common tool in assisted reproductive laboratories. Oxidative stress during freezing process is a major factor of sperm defect after this procedure. Despite of the fact that antioxidant compounds have an effective role on semen freezing techniques, the aim of this study was to evaluate the antioxidant effect of Calligonum on cryopreserved human sperm parameters. Materials and Methods: 10 normozoospermic patients participated in this study. Semen inclusion criteria were as follows: concentration, >60x106/ml; motility, >50%; total motile sperm count, >40x106 and strict morphology score, >10%. First of all, semen samples were frozen. Then, semen samples were washed ones with Ham's F-10 medium +10% HAS, after 24 hours of freezing. After washing, the sperm suspensions were incubated for 1 h at 37°C in medium supplemented with 10 μM/ml concentration of Calligonum extract. Morphology percent, swim up sperm count, total and forward motility of the spermatozoa were measured using computer-aided semen analyzer (CASA). Data was analyzed by one way ANOVA test. Results: The total and forward motility were significantly (p⩽0.05) higher in sperm swim up with the antioxidant (10 μM/ml) than in the control group. Data didn't show any significant difference between two groups in count and morphology percent. Conclusion: Our data reveled that CALLIGONUM extract can improve motility rate. This is an important factor in ART and affect on embryo quality. These changes are related to antioxidant properties of CALLIGONUM extract.


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