BSO-induced oxidative stress affects testicular histology, semen parameters and sperm fertility

Rad, J. Soleimani; Sajjadian, F.; Roshangar, L.; Alihemmati, A.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p117
Academic Journal
Introduction: GSH is the main intracellular antioxidant and its level is important for protecting cell from oxidafive stress. BSO inhibits GSH synthesis and cause oxidative stress. The study is designed to investigate the effect of BSO induced oxidative stress on testicular histology, semen parameters and sperm fertility. Materials and Methods: The adult male mice were divided into two groups of control and experiment. In the experimental group the mice received 2mmol /kg BSO for 35 days as IP injection. All mice were sacrificed at the end of the study and their testes were prepared for histological studies. For determination of semen parameters, sperms were collected from cauda epididymis and analyzed under light microscope. For evaluation of fertility the oocytes were obtained from superovulated mice by tubal flashing and inseminated with sperms. Embryo formation was considered as fertility success and compared in between groups. Results: Semen Analysis revealed that in experimental group the percent of sperms with progressive motility was less than the control group and the percent of sperms with abnormal morphology was higher than in control group (p<0.05). Histomorphometric studies showed that the percent of Tubal Differentiation Index (TDI) and spermatogenic Index (SI) in experimental group were reduced (p<0.01). Microscopic studies revealed that experimental group had undergone vacuolar degeneration and nuclear condensation of spermatogenic cells. Sperm fertility, based on embryo formation, was decreased in experimental group. Conclusion: A morphological change of mice testes in Bso-induced oxidafive stress is accompanied by decreasing of TDI and SI values and fertility rate.


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