Nano-particle Titanium Dioxide (TiO2)-induced damages in mice testis correlates with enzymatic antioxidant line deficiency

Teyri, V.; Razi, M.; Farokhi, F.; Shafiee, S.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p117
Academic Journal
Introduction: Nano-particle Titanium Dioxide (TiO2) is a non-combustible, odorless powder that is widely used for sunscreens and as coating for self-cleaning windows. There are several reports, which are indicating that TiO2 is able to exert cytotoxic impacts and induce lipid peroxidation and severe DNA damage. In present study we examined the dose effect of TiO2 on germinal cells apoptosis as well as its impact on enzymatic antioxidant status. Materials and Methods:Twenty four mature mice were randomly divided into four groups as control-sham (received 0.3mL normal saline, ip) and test groups including: low dose (10 mg/kg, ip), medium dose (50 mg/kg, ip) and high dose (100mg/kg, ip)-TiO2-received groups. After 35 days, the tissue levels of glutathione peroxidase (GSH-px), super oxide dismutase (SOD), and catalase (CTS), the serum level of testosterone and Leydig cells steroidogenic foci were evaluated. Germinal cells apoptosis was estimated using TUNEL staining. Results: TiO2 significantly (p<0.05) decreased GSHpx, SOD and catalase levels of the testicles in a dose dependent manner. Epi-fiuorescent analyses showed that the intracytoplasmic steroid content of the Leydig cells decreased in TiO2-received animals and developed depending on dose. In a same pattern, the serum level of testosterone decreased and percentage of tubules with apoptotic cells enhanced depending on dose. Conclusion: Our data suggest that, TiO2 exerts its pathological impact via down-regulating antioxidant status, which in turn is able to adversely impact the endocrine status. Ultimately defected antioxidant capacity associated with damaged endocrine status provokes the apoptosis in germinal cells.


Related Articles

  • GADD45β plays a protective role in acute lung injury by regulating apoptosis in experimental sepsis in vivo. Moon, Sung‐Je; Kim, Hyun‐Yong; Kim, Yong‐Hoon; Kim, Kyoung‐Shim; Noh, Jung‐Ran; Kim, Hyun‐Jong; Choi, Ji‐Hyun; Hwang, Jung H.; Lee, Chul‐Ho // Journal of Cellular Physiology;Oct2018, Vol. 233 Issue 10, p7128 

    Sepsis is a systemic inflammatory response syndrome due to microbial infection. Growth arrest and DNA‐damage‐inducible 45 beta (GADD45β) are induced by genotoxic stress and inflammatory cytokines. However, the role of GADD45β during bacterial infection remains unclear....

  • In vivo single-photon emission computed tomography imaging of apoptosis in Crohn's disease and anti-tumour necrosis factor therapy. Watson, Alastair J. M. // Gut;Apr2007, Vol. 56 Issue 4, p461 

    The article reports on a study by J. M. H. Van der Brande and colleagues on evidence for the importance of apoptosis of lamina propria T cells in the action of infliximab. The researchers demonstrate that apoptosis in the intestine is increased compared with uninflammed intestine in two mouse...

  • Galectin-9 in Allergic Airway Inflammation and Hyper-Responsiveness in Mice. Sziksz, Erna; Kozma, Gergely Tibor; Pállinger, Éva; Komlósi, Zsolt István; Ádori, Csaba; Kovács, Lajos; Szebeni, Beáta; Rusai, Krisztina; Losonczy, György; Szabó, András; Vannay, Ádám // International Archives of Allergy & Immunology;2010, Vol. 151 Issue 4, p308 

    Background: Galectin-9 (Gal-9) is a member of the growing family of β-galactoside-binding lectins. Gal-9 is an eosinophil chemoattractant and inducer of Th1 cell apoptosis. These effects suggest its potential role in the pathogenesis of asthma. Our aim was to study the expression of Gal-9 in...

  • Baicalin Improves Survival in a Murine Model of Polymicrobial Sepsis via Suppressing Inflammatory Response and Lymphocyte Apoptosis. Jiali Zhu; Jiafeng Wang; Ying Sheng; Yun Zou; Lulong Bo; Fei Wang; Jingsheng Lou; Xiaohua Fan; Rui Bao; Youping Wu; Feng Chen; Xiaoming Deng; Jinbao Li // PLoS ONE;May2012, Vol. 7 Issue 5, p1 

    Background: An imbalance between overwhelming inflammation and lymphocyte apoptosis is the main cause of high mortality in patients with sepsis. Baicalin, the main active ingredient of the Scutellaria root, exerts anti-inflammatory, antiapoptotic, and even antibacterial properties in...

  • Retinal Cell Death Caused by Sodium Iodate Involves Multiple Caspase-Dependent and Caspase-Independent Cell-Death Pathways. Balmer, Jasmin; Zulliger, Rahel; Roberti, Stefano; Enzmann, Volker // International Journal of Molecular Sciences;Jul2015, Vol. 16 Issue 7, p15086 

    Herein, we have investigated retinal cell-death pathways in response to the retina toxin sodium iodate (NaIO3) both in vivo and in vitro. C57/BL6 mice were treated with a single intravenous injection of NaIO3 (35 mg/kg). Morphological changes in the retina post NaIO3 injection in comparison to...

  • VDAC activation by the 18 kDa translocator protein (TSPO), implications for apoptosis. Leo Veenman; Yulia Shandalov; Moshe Gavish // Journal of Bioenergetics & Biomembranes;Jun2008, Vol. 40 Issue 3, p199 

    Abstract  The voltage dependent anion channel (VDAC), located in the outer mitochondrial membrane, functions as a major channel allowing passage of small molecules and ions between the mitochondrial inter-membrane space and cytoplasm. Together with the adenine nucleotide translocator...

  • Testicular germ cell apoptosis and sperm defects in mice upon long‐term high fat diet feeding. Ghosh, Songita; Mukherjee, Sutapa // Journal of Cellular Physiology;Oct2018, Vol. 233 Issue 10, p6896 

    The growing prevalence of male infertility is a matter of serious concern. One of the putative causes being nutritional excess from continuous consumption of high fat diet (HFD) leading to insulin resistance albeit the specific relationship is not fully understood. Besides, there are many...

  • Atrazine-induced apoptosis of splenocytes in BALB/C mice.  // BMC Medicine;2011, Vol. 9 Issue 1, p117 

    The article focuses on a study which investigates the apoptosis of splenocytes in mice exposed to atrazine (ATR). For 21 days, oral doses of ATR were administered to BALB/C mice. Examination of the histopathology, lymphocyte apoptosis and the expression of apoptosis-related proteins from the...

  • Reduced levels of SCD1 accentuate palmitate-induced stress in insulin-producing β-cells. Thörn, Kristofer; Hovsepyan, Meri; Bergsten, Peter // Lipids in Health & Disease;2010, Vol. 9, p108 

    Background: Stearoyl-CoA desaturase 1 (SCD1) is an ER resident enzyme introducing a double-bond in saturated fatty acids. Global knockout of SCD1 in mouse increases fatty acid oxidation and insulin sensitivity which makes the animal resistant to diet-induced obesity. Inhibition of SCD1 has...

  • Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria. Wiese, Lothar; Hempel, Casper; Penkowa, Milena; Kirkby, Nikolai; Kurtzhals, Jørgen A. L. // Malaria Journal;2008, Vol. 7, Special section p1 

    Background: Cerebral malaria (CM) is an acute encephalopathy with increased pro-inflammatory cytokines, sequestration of parasitized erythrocytes and localized ischaemia. In children CM induces cognitive impairment in about 10% of the survivors. Erythropoietin (Epo) has -- besides of its well...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics