The detrimental effects of silver nanoparticles on sperm chromatin structure and DNA integrity in mice

Talebi, A. R.; Yavari, M.; Rezaei Zarchi, Z.; Nayeri, M.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p115
Academic Journal
Introduction: Since the sperm chromatin structure and DNA integrity are essential for fertility and regarding the effect of silver nanoparticles on fertility potential of sperm cells, the effects of silver nanoparticle on mouse spermatozoa were evaluated. Materials and Methods: In this experimental study, 24 adult male Syrian mice were divided in a control group and three experimental groups. The 40 nm silver nanoparticles with three different doses of 50, 100 and 200 μl orally administered for 5 weeks. After this time, the cauda epididymal sperms were aspirated for analysis of sperm parameters (count, morphology and motility) according to WHO criteria. The sperm chromatin structure and DNA integrity were assessed using three different cytochemical tests including aniline blue (AB), toluidine blue (TB) and chromomycin A3 (CMA3) test. Results: The third experimental group (highest dose of silver nanoparticles), had significantly the lowest mean of sperm count, the lowest percentage of fast progressive motile spermatozoa, and the highest percentage of abnormal form sperm cells. Also, the evaluation of sperm chromatin condensation and chromatin packaging showed that the study groups II and III had thespermatozoa with lesser condensed chromatin than the other groups (p<0,05). Conclusion: According to the results of this study, the negative effect of silver nanoparticles on sperm parameters and sperm chromatin structure and DNA integrity was observed in mice. In general the toxicity of silver nanoparticles was dose-dependent, so a group of mice that had received the highest dose of nanosilver, had the lowest sperm quality.


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