Many laboratories have no standardization in performing and reporting the semen analysis

Yari, N.; Etebary, S.; Khalili, M.; Nabi, A.; Ghasemnezhad, R.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p114
Academic Journal
Introduction: The semen analysis is the most important clinical laboratory test used in diagnosis of male factor infertility. In practice, the careful analysis of sperm concentration, movement, and morphology requires a great deal of technical expertise and procedural care. Although, World Health Organization (WHO) has published a series of laboratory manuals to perform and report semen analysis, in practice the reported lack of standardization, the wide variation among laboratories bas led some to consider the semen analysis inaccurate and unreliable. Like many countries, in Iran we have the same problems in semen analysis. The aim of this study was to assess semen analysis reports in Iran. Materials and Methods: 141 semen analysis reports were collected and assessed from patients referred to Yazd research & clinical center for infertility during September 2013 to march 2014. Demographic data and reported parameters in semen analysis were compared with recommended WHO standards. Results: None of laboratories reported duration of abstinence, time and type of sample collection. Only 22% of reports had macroscopic parameters of semen analysis. In microscopic parameters section, normal range was mentionedin 51 % of reports. No laboratory reported type of counting chamber and method of sample staining for morphology. Non-progressive motility and immotile sperms were not reported in 9.9% and 17% of reports, respectively. 1% of reports were completely qualitative. In none of the reports, WHO guideline edition was noted. Conclusion: It is strongly recommended that laboratories performing the semen analysis adopt accepted normal range of parameters such as those proposed by WHO.


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