Effects of L-carnitine and Pentoxifylline on the activity of Lactate Dehydrogenase C4 isozyme and motility of testicular spermatozoa in mice

Aliabadi, E.; Karimi, F.; Rasti, M.; Akmali, M.; Esmaeilpour, T.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p113
Academic Journal
Introduction: Extracted sperm from the testis have poor motility. Moreover, their motility changes during their journey through epidydimis. Meanwhile, they face high concentration of L-carnitin. In addition, lactate dehydrogenase C4 (LDH-C4) gene disorders has been shown to cause impaired sperm motility, leading to infertility in male mice. The aim of this study was to evaluate sperm motility and LDH-C4 enzyme activity upon L-camitine (LC) and Pentoxifyiline (PTX) administrations in mice. Materials and Methods: We extracted testicular sperm of 48 mice and divided them into three equal parts. One part was incubated with Ham's FlO medium (control), the other parts were treated with Ham's FlO containing LC and PTX with a final concentration of 1.76 mM, for 30 min at room temperature. Sperm mofility was assessed according to the World Health Organization (WHO) criteria. Sperm LDH-C4 enzyme activity was measured by spectrophotometery method. Statistical analyses were performed using ANOVA and Fisher's LSD test, and a p-value less than 0.05 was considered as a statistically significant difference. Results: Sperm motility increased after 30 min of incubation in LC- and PTX-treated group (p<0.001). LC and PTX administrations showed a significant increase in the LDHC4 enzyme activity of sperm compared to that of the controls after 30 min (p=0.04 and 0.01, respectively). Conclusion: The effects of LC and PTX on motility of sperm can be explained by an increase in LDH-C4 enzyme activity that may influence male fertility status. We suggest that LC as a non-toxic antioxidant is more suitable for use in assisted reproductive technique protocols than PTX.


Related Articles

  • Things To Come: France Will Soon Disappear. SILVERSTEIN, MICHAEL // Moderate Voice;12/6/2012, p11 

    The article reports on a study which found that the average sperm count among Frenchmen, aged 18 to 70, between 1989 and 2005, fell more than 32% in this period. This is still above the infertility level, however, the trend is clear. The large unoccupied area will leave between Germany and Spain...

  • TRYING TO GET PREGNANT?  // Good Health (Australia Edition);Jun2012, p24 

    The article offers information on new research which found that men who consumed the most dietary fats had a 43 per cent lower total sperm count than those who consumed the least.

  • Conference Abstracts ... January 5, 2015.  // Fertility Weekly;1/5/2015, p13 

    Several abstracts of papers presented at the 62nd Annual Meeting of the Pacific Coast Reproductive Society in Indian Wells, California held during March 19-23, 2014 are presented including Effects of Male Tobacco Use on Both Total Motile Sperm Count and Pregnancy Rates in Intrauterine...

  • Study Suggests Long-Term Decline in French Sperm Quality. Burton, Adrian // Environmental Health Perspectives;Feb2013, Vol. 121 Issue 2, pA46 

    The article presents information on a study, according to which there has been a considerable decline in sperm quality of French men between 1989 and 2005, and the average sperm count is falling. However, the researchers said that there has been a selection bias, as most studies have relied on...

  • TV A DANGER TO MEN.  // Natural Solutions;May2013, Issue 153, p12 

    The article focuses on a research according to which men who watch television for more than 20 hours a week have 44 percent lower sperm count as sitting on the couch for multiple hours increases the temperature of testicles and damages their sperm.

  • Editorial. Adeyemi, Joseph; Ejike, Chukwunonso; Jimoh, Florence; Hui Li; Nianwei Lin; Omonkhua, Akhere; Zhuoxin Yu // Biokemistri;8/31/2013, Vol. 25 Issue 2, p24 

    An introduction is presented in which the editor discusses various reports within the issue on topics including the spermatogenic effect of honey for testosterone production, the use of garlic and ginger as natural food preservatives and the aquatic environmental pollutants.

  • Sperm Count. Hall, Jim // North American Review;Mar83, Vol. 268 Issue 1, p63 

    Presents the poem "Sperm Count," by Jim Hall.

  • Long-term Effects of Betamethasone on Epididymal Tissue, Epididymal Sperm Counts and Fertility in Male Mice. Gouyandeh, Jalaleddin; Modaresi, Mehrdad; Mansouri, Sara; najafabadi, Fahimeh Yaghoubieh // Journal of Chemical Health Risks;2015, Vol. 5 Issue 4, p295 

    Recent studies show that synthetic glucocorticoids alter testicular homeostasis, In this regard, the influence of Betamethasone as a glucocorticoid widely used on histological changes, epididymal sperm counts and fertility was explored in male mice. The study sample (50 mice) was allocated to 3...

  • Effects of vitamin E on antispermatogenic activity of alcohol in male albino rats. Akintayo, C. O.; Olaniyan, O. T. // Proceedings of the Physiological Society;2013, p708P 

    Several factors affect fertility, ranging from environmental factor, nutritional factor, toxins and the way of life. However, Alcohol is often associated with fertility disturbances with low sperm count and impaired sperm motility. The sertoli cell seems to be the first testicular cell injured...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics