TITLE

Evaluating the spermicidal activity of a new antimicrobial peptide from the Insect Paederus dermatitis, Sarcotoxin Pd

AUTHOR(S)
Fesahat, F.; Zare-Zardini, H.; Anbari, F.; Ebrahimi, L.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p111
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: The aim of this study was to evaluate the spermicidal activity of Sarcotoxin Pd as new cationic antimicrobial peptides (AMPs) with antimicrobial properties from the Insect Paederus dermatitis. Materials and Methods: The purifled Sarcotoxin Pd was diluted with Ham's FIO in 40, 50, 60, 70, 80 and 90(μg/ml accordingly. 1 ml from peptide solution with different dosage was mixed with 200μl prepared sperm solution in microtube. Sperm motility, viability and morphology were assessed at different time intervals (0.3, 5, 10, 15 min). Also, the potential cytotoxicity of sarcotoxin Pdagainst normal human cervical cell (HeLa) was measured by the MTT (3-[4, 5-dimethylthiazol-2- yl]-2, 5-diphenyI tetrazolium bromide)-based assay. Eosin-Nigrosin staining and Giemsa staining methods were applied for sperm viabilify and morphology detection, respectively. Also, for statistical analysis, repeated measure was used. Differences were considered statistically signiflcant if p<0.05. Results: This compound immobilized sperm with its effective dose only within 20 s (EC 100) at a concentration of 90 μg/mL and spermicidal activity was time-dependent without any morphological change in the sperm head, midpiece or tail. Also, the cytotoxic effect of sarcotoxin Pd was concentration-dependent and signiflcant cytotoxicity of this peptide was observed at concentrations higher than 24 μg/ml. Conclusion: Sarcotoxin Pd has spermicidal activity in addition to antimicrobial activities. It seems this peptide might be a potential candidate in order to use in male contraception, although, this preliminary study needs more studies to elucidate final conclusion.
ACCESSION #
96841763

 

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