Phenylhydrazine-induced anemia in mice and its effect on testicular tissue and sperm parameters; evidence for protective effect of royal Jelly versus vitamin C

Anbara, H.; Shahrooz, R.; Razi, M.; Malekinejad, H.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p110
Academic Journal
Introduction: Phenylhydrazine (PHZ) is used to prepare indoles and to form phenylhydrazines of natural mixtures of simple sugars. However, it is used to induce experimental anemia in animal models. On the other hand royal jelly is known as antioxidant compound. Therefore, here in present study we aimed to evaluate the protective effect of royal jelly on PHZ-induced damages at testicular and sperms level and compare its impact with vitamin C. Materials andMethods: Eighteen mature male mice were randomly divided into 3 groups. In order to induce experimental anemia, PHZ was administrated at dose of 8 mg/kg, ip and then it was continued as 6 mg/kg, every 48 hours, ip in all groups. The test groups subdivided into non-treated PHZ-received, royal jelly-received (100 mg/kg, orally) and vitamin C-received (250 mg/kg, ip). After 35 days, the tubular differentiation (TDI), spermiogenesis (SPI) indexes were evaluated. Sperm count; motility and DNA integrity were analyzed by using light and fluoreseent microscopes, respectively. Results: Royal jelly reduced PHZ-increased percentage of tubules with negative TDI and SPI. No significant differences were observed between royal jelly and vitamin C. Comparing the sperm parameters showed that royal jelly similar to vitamin C (P<0.05) improved sperm count, motility and reduced sperm DNA damage. Conclusion: Our data showed that royal jelly exerts similar effects like vitamin C as an antioxidant agent and can be considered as an appropriate compound in order to inhibit anemia-induced damages at testicles and sperm levels.


Related Articles

  • Testicular microlithiasis: Is there an agreed protocol? Shanmugasundaram, R.; Singh, J. Changra; Kekre, Nitin S. // Indian Journal of Urology;Jul-Sep2007, Vol. 23 Issue 3, p234 

    This review addresses the issues on etiopathogenesis of testicular microlithiasis (TM), associated clinical entities, evaluation and follow-up of patients with TM. A literature search of Medline/PubMed was carried out using the keywords 'testicular microlithiasis' and 'testicular calcifications'...

  • Testicular pain common after lap nephrectomy. Guttman, Cheryl // Urology Times;Feb2008, Vol. 36 Issue 2, p16 

    The article reports on the study conducted by urologists from Indiana University School of Medicine in Indianapolis, Indiana, which warned men undergoing laparoscopic nephrectomy about the risk in developing testicular pain postoperatively. The researchers analyzed prospectively collected data...

  • Risk Factors for Chronic Kidney Disease Progression Identified.  // Renal & Urology News;Sep2015, Vol. 14 Issue 7, p16 

    The article focuses on the risk factors for chronic kidney disease (CKD) progression which include high blood pressure, anemia, and proteinuria.

  • Aplastic anemia in a petrochemical factory worker. Baak; Byoung Yong Ahn, Young Mann // Environmental Health Perspectives;Oct1999, Vol. 107 Issue 10, p851 

    Describes aplastic anemia in a petrochemical factory worker who had been exposed to low-level benzene during the production and handling of petroleum resin products. Relation of amount and duration of exposure to hematoxicity of benzene; Risk assessment studies; Need for further research to...

  • Stage I testicular seminoma: Surveillance versus adjuvant radiotherapy. Berkmen, Ferhat; Hizli, Fatıh // Turkish Journal of Cancer;Oct2006, Vol. 36 Issue 4, p169 

    Despite optimal radiation field is still being discussed, the traditional treatment for patients with low-stage seminoma of the testis has been orchiectomy followed by external-beam radiation therapy. The other treatment modalities are adjuvant radiation therapy and/or chemotherapy. We sought to...

  • Testicular microlithiasis as a familial risk factor for testicular germ cell tumour. Coffey, J.; Huddart, R. A.; Elliott, F.; Sohaib, S. A.; Parker, E.; Dudakia, D.; Pugh, J. L.; Easton, D. F.; Bishop, D. T.; Stratton, M. R.; Rapley, E. A. // British Journal of Cancer;12/17/2007, Vol. 97 Issue 12, p1701 

    Testicular microlithiasis (TM) is characterised by small intratesticular calcifications, which can be visualised by ultrasound. Men with testicular germ cell tumour (TGCT) have a higher frequency of TM than men without TGCT. To clarify the association between TGCT and TM and to investigate the...

  • A Meta-Analysis of the Relationship between Testicular Microlithiasis and Incidence of Testicular Cancer. Tao Wang; LuHao Liu; JinTai Luo; TaiSheng Liu; AnYang Wei // Urology Journal;Mar/Apr2015, Vol. 12 Issue 2, p2057 

    Purpose: There are many recent observational studies on testicular microlithiasis (TM) and risk of testicular cancer. Whether TM increases the risk of testicular cancer is still inconclusive. The objective of this updated meta-analysis was to synthesize evidence from clinical observational...

  • Heavy Metal Levels in Adolescent and Maternal Blood: Association with Risk of Hypospadias. Sharma, Tusha; Banerjee, Basu Dev; Yadav, Chandra Shekhar; Gupta, Piyush; Sharma, Sunil // ISRN Otolaryngology;2014, p1 

    Background. Hypospadias is a part of testicular digenesis syndrome (TDS) which includes infertility, cryptorchidism, and spermatogenesis. Heavy metals act as endocrine disrupting compounds. Heavy metals such as cadmium, chromium, arsenic, and lead have been associated with male infertility,...

  • Chronic kidney disease and the older person. Kane-ToddHall, Steve MBJM // Update;Feb2007, Vol. 74 Issue 2, p74 

    The article focuses on the risk of various diseases which is associated with chronic kidney disease (CKD) in older people. CKD results in an increased risk of cardiovascular (CV) diseases. It may cause anemia which requires assessment in the usual fashion. CKD may cause bone disease. The use of...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics