TITLE

Effect of crab shell extract on cell viability and apoptosis of human umbilical vein endothelial cells

AUTHOR(S)
Mirzapur, P.; Rezakhani, L.; Khazaei, M.; Rashidi, Z.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p109
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: Angiogenesis is formation of new blood vessels from pre-existing vessels. There are different therapeutic strategies in researehes for treatment of various diseases and cancers to increase or decrease of vessels growth, including the use of natural components instead of chemicals drugs. The aim of this study was to investigation the effcet of crab shell ethanolic extract on human umbilical vein endothelial cells in vitro. Materials and Methods: In this study, human umbilical vein endothelial cells (HUVECs) were used. After preparation of the crab shell extract, the HUVECs were treated with different concentrations of crab shell (100, 200, 400, 800 and 1000 μg/ml) at 24, 48 and 72 hours. Cell viability by MTT assay and apoptosis by TUNEL assay were assessed. One-way ANOVA was used for data analysis. P<0.05 was considered significant. Results: HUVECs viability was decreased in 24, 48 and 72 h, affected by crab shell extract dose and time dependent manner (p<0.05). After 72 hours, signiflcant increasing in apoptosis was observed. Conclusion: Crab shell has antiproliferative effect on human umbilical vein endothelial cells and exerts this effect by inducing apoptosis.
ACCESSION #
96841757

 

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