TITLE

Mesenchymal stem cells from rat bone marrow and adipose tissue similary to expressed neurotrophic factors in vitro

AUTHOR(S)
Mirzaeiyan, L.; Ghorbanian, M. T.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p108
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: MSCs have been attracted great attention because of their neuroprotective effects both in vitro and in vivo by secreting various factors. Although bone marrow is the most common MSC source, cells with similar characteristics have been shown to be present in several other adult tissues. Adipose tissue, large quantities of which can be easily obtained, represents an attractive alternative to bone marrow in isolating adipose tissue-derived MSC (ADSC). Our purpose was to comparatively examine under appropriate in vitro conditions, expression of some neurotrophic factors such as brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF), glial cell-derived neurotrophic factor (GDNF), neurotrophin-3 (NT-3), neurotrophin-4 (NT-4), nerve growth factor (NGF) and the expression of nestin. Materials and Methods: Isolated ADSCs and BMSCs from the subcutaneous adipose tissue and bone marrow from adult rat subjects, respectively. ADSCs and BMSCs were cultured in a-MEM supplemented with 10% FBS. Reverse transcription-polymerase chain reaction (RT-PCR) was carried out to evaluate the expression of the above-mentioned neurotrophic factors in both cells were in-vitro. Results: The morphological, immunophenotypical and expression of above neurotrophic factors of both types of cells were almost identical in ADSCs and BMSCs. Conclusion: This finding supports Bone marrow and adipose tissue contains a stem cell population which expressed nestin and neurotrophic factors genes, it seems to be a good candidate for the future cell replacement therapy and improvement of neurodegenerative diseases.
ACCESSION #
96841756

 

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