Evaluation of gene expression in Adipose-Derived Mesenchymal Stem Cells using real time PCR method

Ghiasi, M.; hasan, M. Sheykh; Qomi, R. Tabatabaei; Fazaely, H.; Kalhor, N.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p106
Academic Journal
Introduction: Mesenchymal stem cells (MSCs) are non-specialized cells that are found in many adult and embryonic tissues. These cells have the huge self renewal potential and ability to differentiate into many kinds of cell lineages in vitro. Adipose tissue is as one of the important tissues used to obtain these cells. Mesenchymal stem cells derived from adipose tissue have the pluripotent characteristics, reproducibility and plasticity. They also have high repair potential as a promising approach for the treatment of diseases in tissue engineering and regenerative medicine. Materials and Methods: In this study, mesenchymal stem cells were isolated from human subcutaneous adipose tissue and these cells can be expanded in vitro and reach to desired number. The expression of collagen I, collagen II, aggrecan, and sox9 in these cells were analyzed by Real time PCR method. Results: Based on the results of this study, collagen I & II expression aggrecan and sox9 were very low in MSCs. Conclusion: Theses results indicated that mesenchymal stem cells have an undifferentiated state.


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