Characterization of mesenchymal stem cells from human umbilical cord Wharton's jelly: potential differentiation of germ-like cells in vitro

Maghami, R. Ghaem; Abdollahi, Z.; Najafzadeh, N.; Bayrami, A.; Zahri, S.; Mirzapour, T.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p101
Academic Journal
Introduction: For humans, cytotoxic drug therapy to eradicate cancer cells can damage spermatogenesis and lead to infertility. Theoretically, isolation and differentiation of MSC to germ-like cells could provide a source of endogenous germ cells for possible transplantation for patients undergoing chemotherapy in future. Materials and Methods: Pluripotent mesenchymal stem cell (MSC) lines were established from umbilical cord Wharton's jelly. The cells present the high expansion potential of MSC, a typical fibroblast-like morphology and proliferate up to 15 passages without displaying clear changes in morphology. PCR analysis was performed on MSCs at passages 2-5 to further characterize this cell population. The results showed that these cells strongly expressed markers for mesenchymal progenitors such as CD 105 and CD44 and were negative for cell surface markers associated with hematopoietic cells, CD34. MSC formed tadpolelike germ cells after induction with different dosages of retinoic acid and conditioned medium. Results: The present study showed that, mesenchymasl stem cells are fully functional after proliferation and culturing process do not damages the surface properties of these cells. The ability to isolate and culture of MSCs in vitro and to apply transplantation technique may facilitate development of therapeutic strategies for the treatment of infertility in patients who may suffer destruction of germ cells as a result of irradiation or chemotherapy for cancer. Conclusion: Our results may provide a new route for reproductive therapy and a novel in vitro model to investigate the molecular mechanisms that regulate the development of the mammalian germ lineage.


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