Pulegone-induced damages in mice ovarian tissue correlates with CYP19 gene expression

Souldouzi, R.; Razi, M.; Shalizar, A.; Amani, S.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p100
Academic Journal
Introduction: Pulegone is a naturally occurring organic compound in variety of plants that is widely used for flavoring foods, drinks, and dental products. However, there are several reports indicating its cytotoxic effect on various cell types. The CYP19 is an enzyme catalysing the conversion of androgens into oestrogens. Therefore, present study was conducted in order to uncover the effect of ±pulegune on CYP19 mRNA expression and its impact on follicular growth in mice model. Materials and Methods: Twenty four mature female mice were divided into control-sham (received 0.5 mL normal saline, orally) and test groups including; 25mg/kg, 50mg/kg and 100mg/kg pulegone-treated groups. The mRNA level of CYP19 was assessed by semiquantitative-PCR. Total follicle and atretic follicles distribution per ovary and serum level of estradiol were evaluated. Results: Pulegone reduced CYP19 expression in a dose dependent manner. Pulegone-received animals exhibited significantly (p<0.05) lower follicle distribution and remarkably (p<0.05) higher number of atretic follicles versus control-sham groups. Depending on dose, the serum level of estradiol decreased in pulegone-treated animals. Conclusion: In conclusion, our data showed that pulegone exerts its pathological impact by decreasing oestrogen synthesis via down-regulating CYP19 expression. Ultimately, pathologically altered oestrogen synthesis provokes follicular atresia.


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