The effect of Vitex agnus castus on structure and volume of corpus luteum in mice

Hamidian, Gh.; Yahyavi, F.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p96
Academic Journal
Introduction: Vitex agnus-castus L. (VAC) is a phytosterogen and has been used traditionally in the treatment of menstrual disorders, premenstrual syndrome and corpus luteum insufficiency but there is no scientific evidence about its effect on corpus luteum. So, this study was aimed to evaluate the effects of VAC essential oil on structure and volume of corpus luteum in mice by unbiased stereological technique. Materials and Methods: In this study 20 young adult female BALB/c mice were randomly divided into four groups: group 1 as control group and group 2, 3 and 4 which received 75, 150 and 300 mg/kg VAC essential oil via gastric gavage for seven consecutive days, respectively. At the eighth day, animals were euthanized and the ovaries were quickly removed, weighted and fixed in 10% buffered formalin. The samples were processed by routine and standard paraffin embedding and using the isotropic uniform random samples were sectioned. Eight to twelve sections from each ovary were sampled through systematic random sampling and were stained by H&E. The volume and volume density of corpus luteum were estimated by Cavalieri's method. Results: The results showed that volume and volume density of corpus luteum were increased in all treated animals compared to control group. This dose-dependent increasing was not significant in group 2 but there is a significant differences between group 3 and 4 compared to control (p<0.05). Conclusion: Our findings indicated that VAC essential oil as a phytosterogen can affect structure and size of corpus luteum in a dose dependent manner.


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