TITLE

The role of Selenium in optimizing different parameters of human sperm after cryopservation process

AUTHOR(S)
Amidi, F.; Rezaeian, Z.; Moghbelinejad, S.; Fallahi, P.; Safdari, L.; Nassiri-asI, M.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p95
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: Semen cryopreservation is an important procedure which is used frequently before assisted reproductive techniques (ART). Sperm cryopreservation and thawing is associated with increased ROS production and decreased antioxidant level. In this research we evaluated the effects of the Selenium, as an antioxidant, on some human sperm parameters after cryopreservation process. Materials and Methods: Sperm samples were obtained from 43 men referring to the Fertility and Infertility Center of Shariati Hospital (Tehran, Iran). After the sperm parameters were determined according to World Health Organization (WHO) guidelines, samples were divided into two parts: one without Se, as control and the other one with 5ug/ml Se. Control and treated specimens were then cryopreserved by the liquid nitrogen vapor method. After two weeks interval, the samples were thawed at room temperature, and then sperms concentration, motility, viability, morphology and DNA damage were evaluated by different methods. Results: Our results showed that Se significantly increased sperms concentration, motility, viability and decreased the rate of sperms DNA damage in treated samples, compared to control ones (p<0.05). Conclusion: This study suggests that adding Se to sperm sample as an antioxidant, before cryopreservation, protects sperms from mortal effect of freezing procedures, therefore Se can be used in infertility clinics.
ACCESSION #
96841721

 

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