TITLE

Effect of oxidative stress on testis histopathology in mature male mouse

AUTHOR(S)
Fatemi, ,3N.; Gourabi, H.; Sanati, M. H.; Moayer, F.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p94
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: Approximately 10-15 percent of couples are infertile. Oxidative stress and Reactive oxygen species (ROS) is one of the main reasons of male infertility. In this study, the effect of oxidative stress induced by tertiary-butyl hydroperoxide (TBHP) was investigated on testis histopathology in mouse. Materials and Methods: TBHP was intraperitoneally (ip) injected at the dilution of 1:10 median lethal dose (LD50) into the adult male mice strain Balb/c for two weeks. In continuous, the mice were sacrificed and testis tissues were removed in order to assay the ROS level and Histological analysis. Results: The flow cytometry analysis showed a significant increase in ROS level in testis. Testis histopathology following TBHP treatment revealed a significant decrease in Leydig and germ cells, seminiferous tubule diameter, germ layer thickness, but interstitial connective tissue thickness showed an increase. In addition, germ cells disorganization and progressive degeneration were detected. Conclusion: In conclusion, oxidative stress induced by TBHP could lead to the various morphological changes in seminiferous tubules and also play a potential role in the pathogenesis and protective mechanisms of testis.
ACCESSION #
96841717

 

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