TITLE

In vitro maturation of human oocytes using supernatants of mesenchymal stem cells and embryo formation by use of ICSI

AUTHOR(S)
Fazaely, H.; hasan, M. Sheykh; Qomi, R. Tabatabaei; Kalhor, N.; Ghiasi, M.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p93
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: In vitro maturation of oocytes can reduce costs and avoid side effects gonadotropin stimulation produced in ivf cycle. Unfortunately synthetic maturation mediums are not efficient. The aim of this study was to find an approach to increase fertility. Molecules secreted by mesenchymal stem cells into the culture medium can resume meiotic division. In this study it was demonstrated that the supernatants of mesenchymal stem cells could provide a suitable environment for maturation of oocytes whereas matured oocytes are able to reach blastocyst stage. Materials and Methods: Oocytes in germinal vesicle stage were placed into commercial medium and supernatant of mesenchymal stem cells that consist of DMEM 10% (supplemented with FBS). After 48 hours, oocytes were observed using an invert microscope to detect the release of polar body as a sign of oocyte maturation. Results: According to obtained data and by use of chi square test about maturating GV oocytes the difference between these two used medium was not significant but about embryo formation, they differed significantly (p<0.05). Conclusion: The obtained results showed that supernatant of mesenchymal stem cells in comparison with commercial medium was more efficient and effective, and didn't disurb subsequent embryonic development of matured oocytes.
ACCESSION #
96841716

 

Related Articles

  • Culture, identification and compaction of in vitro cultured adipose-tissue derived and bone marrow mesenchymal stem cells. Mirzaeiyan, L.; Ghorbanian, M. T. // Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p109 

    Introduction: Mesenchymal stem cells (MSCs) have been characterized as multipotent cells which are able to differentiate into several mesodermal and this feature along with their extensive growth and comprehensive immunomodulatory properties establish them as a promising tool for therapeutic...

  • Stimulation of embryo hatching and implantation by prostacyclin and peroxisome proliferator-activated receptor {delta} activation: implication in IVF. J.-C. Huang; W.-S.A. Wun; J.S. Goldsby; I.C. Wun; D. Noorhasan; K.K. Wu // Human Reproduction;Mar2007, Vol. 22 Issue 3, p807 

    BACKGROUND: Successful IVF depends in part on quality embryos. Recent work suggests that prostaglandin I2 (PGI2 or prostacyclin) promotes the development of embryos in vitro and enhances their implantation potential. The mechanism underlying the effects of PGI2 is unclear. It has been reported...

  • Differentiation potential of male mouse adipose tissue derived-mesenchymal stem cells into germ cells. Hosseinzadeh Shirzeily, M.; Pasbakhsh, P.; Mehrannia, K.; Amidi, F.; Sobhani, AGh. // Iranian Journal of Reproductive Medicine;AprSupp, p76 

    Introduction: Recent publications regarding to differentiation of stem cells to germ cells have motivated researchers to make new approaches to infertility. In vitro production of germ cells not only provides new approaches to infertility, but also improves understanding differentiation process...

  • Female Age Affects the Mesenchymal Stem Cell Characteristics of Aspirated Follicular Cells in the In Vitro Fertilization Programme. Virant-Klun, Irma; Omejec, S.; Stimpfel, M.; Skerl, P.; Novakovic, S.; Jancar, N.; Vrtacnik-Bokal, E. // Stem Cell Reviews & Reports;Aug2019, Vol. 15 Issue 4, p543 

    Aspirated follicular cells (AFCs) from the in vitro fertilization program can express various stem cell markers and are even able to differentiate into different types of cells in vitro. The female reproductive potential decreases with increasing age due to lowered ovarian reserve and oocyte...

  • Recipients are at Higher Risk of Pregnancy-Induced Hypertension.  // Fertility Weekly;4/30/2007, p6 

    The article presents a study that investigates the rate of obstetric outcomes and pregnancy-induced hypertension (PIH) in oocyte donation recipients (OD). Data were analyzed from 199 OD recipients and 488 conventional in vitro fertilization (IVF) patients whose ages ranges from 35 to 40 years...

  • Combining uFSH and rFSH Improves Oocyte Maturity and Embryo Cleavage.  // Fertility Weekly;12/24/2007, p6 

    The article discusses the effects of the combination of urinary FSH (uFSH) and recombinant FSH (rFSH) on ovarian stimulation in improved maturity and embryo cleavage. Accordingly, controlled ovarian hyperstimulation (COH) is a fundamental part of in vitro fertilization (IVF) and embryo transfer...

  • Relationship between Serum Polyunsaturated Fatty Acids and Pregnancy.  // Fertility Weekly;12/9/2013, p7 

    The article presents the study "Relationship between serum polyunsaturated fatty acids and pregnancy in women undergoing in vitro fertilization" by Jungheim and colleagues. The authors examined the relationship between serum levels of polyunsaturated fatty acids (PUFAs) and embryo implantation...

  • Extended in Vitro Maturation is not recommended.  // Fertility Weekly;2/14/2011, p3 

    The article presents the findings of a study conducted by U.S. researcher David E. Reichman and colleagues which examines the effects of 24-hour in vitro maturation (IVM) of cumulus-stripped immature oocytes from stimulated cycles. The study revealed that such immature oocytes give rise to...

  • Coats of preimplantation mammalian embryos as a target of reproductive technologies. Rozhkova, I.; Brusentsev, E.; Amstislavsky, S. // Russian Journal of Developmental Biology;Sep2012, Vol. 43 Issue 5, p249 

    The structure and function of the mammalian oocyte and preimplantation embryo coverings are described in this review. The integrity of embryonic coverings is the main prerequisite for the success of such technology as preimplantation embryo freezing and, especially, for successful rederivation....

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics