In vitro maturation of human oocytes using supernatants of mesenchymal stem cells and embryo formation by use of ICSI

Fazaely, H.; hasan, M. Sheykh; Qomi, R. Tabatabaei; Kalhor, N.; Ghiasi, M.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p93
Academic Journal
Introduction: In vitro maturation of oocytes can reduce costs and avoid side effects gonadotropin stimulation produced in ivf cycle. Unfortunately synthetic maturation mediums are not efficient. The aim of this study was to find an approach to increase fertility. Molecules secreted by mesenchymal stem cells into the culture medium can resume meiotic division. In this study it was demonstrated that the supernatants of mesenchymal stem cells could provide a suitable environment for maturation of oocytes whereas matured oocytes are able to reach blastocyst stage. Materials and Methods: Oocytes in germinal vesicle stage were placed into commercial medium and supernatant of mesenchymal stem cells that consist of DMEM 10% (supplemented with FBS). After 48 hours, oocytes were observed using an invert microscope to detect the release of polar body as a sign of oocyte maturation. Results: According to obtained data and by use of chi square test about maturating GV oocytes the difference between these two used medium was not significant but about embryo formation, they differed significantly (p<0.05). Conclusion: The obtained results showed that supernatant of mesenchymal stem cells in comparison with commercial medium was more efficient and effective, and didn't disurb subsequent embryonic development of matured oocytes.


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