Effect of embryonic fibroblast cells conditioned medium on in vitro maturation of immature mouse oocytes

Najafabadi, M. Entezari; Malekshah, A. Karimpour; Moghaddam, A. Esmailnejad; Hamidabadi, H. Ghasemi; Heidari, M.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p92
Academic Journal
Introduction: The purpose of this study was to evaluate the effect of mouse embryonic fibroblast cells conditioned medium on resumption of meiosis, in vitro maturation of immature mouse oocytes, fertilization and derived embryos development. Materials and Methods: Immature oocytes denuded at the geminal vesicle (GV) stage were obtained from female NMRI mice 46-48 hrs after injection of 7/5 IU PMSG. To repartee mouse embryonic fibroblast cells (MEFs), the fetuses were collected from the female mice at day 13 and cultured in DMEM. After the preparation of a monolayer, conditioned medium were collected and used for culture. Immature oocytes were randomly cultured in culture medium of MEM-a supplemented with different concentrations (0, 10, 30, 50 and 100%) of mouse embryonic fibroblast conditioned medium. After 14-16 hrs the matured oocytes were fertilized with spermatozoa in T6 medium and their development was assessed until blastocyst stage. Results: There was no significant differences (p<0.05) in maturation rate between control and conditioned medium groups. But there were significant differences (p<0.05) in the percentage of fertilization and cleavage in conditioned medium with 30%-50% concentrations as compared to the control group (p<0.05) Conclusion: The conditioned medium of fibroblast cells increase in vitro fertilization (IVF) and embryonic development. But there was no effect on the maturation of immature oocytes.


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