TITLE

The protective effect of silymarin on viability, motility and mitochondrial membrane potential of ram sperm treated with sodium arsenite

AUTHOR(S)
Eskandari, F.; Momeni, H. R.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p92
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: Sodium arsenite, as an environmental pollutant, which accumulates in reproductive organs can exert oxidative stress on spermatogenesis and sperm morphology. Due to the antioxidant role of silymarin (an effective extracted from the seeds of Silybum marianum or milk thistle), this study was performed to investigate if silymarin can prevent the adverse effect of sodium arsenite on viability; motility and mitochondrial membrane potential of ram sperm. Materials and Methods: Epidydimal sperm obtained from Farahani's ram (Ovis Aries) was swim up and divided into three groups: 1.control 2.spermatoza treated with sodium arsenite 10 micromolars 3.spermatoza treated with silymarin 20 micromolar + sodium arsenite 10 micromolar and incubated at 37 °C for three hours. MTT assay and Rhodamine 123 were respectively used to assess sperm viability and mitochondrial membrane potential. Sperm motility was done according to World Health Organization (WHO) guidelines. Data were analyzed with one way ANOVA and p<0.05 was considered significant. Results: Viability, motility and intact mitochondrial membrane potential of the spermatozoa were significantly decreased compared to the control group. In silymarin+ sodium arsenite group, silymarin could significantly compensate sperm viability, motility and intact mitochondrial membrane potential decreases compared to sodium arsenite group. Conclusion: Sodium arsenite decreases ram sperm viability, motility and intact mitochondrial membrane potential and silymarin is able to compensate the adverse effect of sodium arsenite on these sperm parameters.
ACCESSION #
96841712

 

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