IVF outcome after using anti freeze protein in semen freezing of three Iranian ovine breeds

Sarvari, A.; Borjian Boroujeni, S.; Naderi, M.; Heidari, B.; Behzadi, B.; Akhondi, M.; Shirazi, A.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p91
Academic Journal
Introduction: Despite the effects of antifreeze proteins (AFPs) on freezing point and their potential interaction with membranes, there are controversial reports on their effects on fertility in different species. In this study the effects of extender supplementation with AFP during semen freezing on embryo development were studied in three Iranian sheep breeds. Materials and Methods: The collected semens from Shal, Afshari, and Moghani ram breeds, before freezing, were diluted in extender supplemented with (Shal-AFP, Afsh-AFP and Mogh-AFP, respectively) or without (Shal-Cnt, Afsh-Cnt and Mogh-Cnt groups, respectively)10μg/ml AFP III. The abattoir-derived COCs were separately fertilized with above frozen semen and the cleavage, blastocyst, and hatching rates were recorded. Results: The highest and lowest blastocyst rates, on day 8, were recorded in shal-Cnt and Afsh-Cnt groups, respectively (26.61±8.58 and 16.61±4.94, respectively; p⩾0.05). The highest and lowest hatching rates were observed in Afsh-Cnt and Mogh- Cnt groups, respectively (55.15±12.06 and 24.61±14.87, respectively; p⩾0.05). Conclusion: Neither the presence or absence of AFP III nor the breeds of rams had effect on the blastocyst formation and hatching rates in in vitro drived embryos.


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