Analysis of TEX14 expression in testis tissues of non-obstructive azoospermic men referred to Royan institute

Borjian Boroujeni, P.; Gourabi, H.; Totonchi, M.; Sabbaghian, M.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p89
Academic Journal
Introduction: Testis-expressed 14 (TEX14) is a novel protein that localizes to germ cell intercellular bridges. During spermatogenesis, proposed roles for the intercellular bridge include germ cell communication, synchronization, and chromosome dosage compensation in haploid cells. In the absence of TEX 14, intercellular bridges are not observed by using electron microscopy and other markers. TEX14 is required for intercellular bridges in vertebrate germ cells, and recent studies provide evidence that the intercellular bridge is essential for sermatogenesis and fertility. Materials and Methods: Testis tissue samples were obtained from 10 patients with severe oligozoospermia and 20 patients with non-obstructive azoospermia (10 with SCOS syndrome and 10 with maturation arrest) who were referred to the Royan institute. Total RNA was extracted with Trizol and cDNA was synthesized. Quantitative real-time RT-PCR was performed using Power SYBR Green kit. Results: Normalizing the relative amount of TEX14 transcript by the amount of GAPDH transcript in the same sample, indicated that expression of TEX 14 in the testis samples of patients with SCOS syndrome (p=0.004) and patients with maturation arrest (p=0.038) is significantly reduced as compared with oligozoospermic patients. Conclusion: The same expression pattern of TEX14 among mouse and human indicated that TEX14 is a highly conserved gene that might has an important role in mammalian testis functions like spermatogenesis. According to the results, we can conclude that TEX14 expression levels are essential for normal spermatogenesis and deficiency in this gene can cause spermatogenic failure and infertility in men.


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