In vitro Effect of crab shell hydroalcoholic extract on apoptosis in breast cancer cells line

Khazaei, M.; Rezakhani, L.; Mirzapur, P.; Rashidi, Z.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p88
Academic Journal
Introduction: Breast cancer is now the most common cancer in women worldwide. According to toxic side effects and drug resistance to chemotherapy, the use of natural compounds with for development of anticancer drugs, has been considered. The aim of the present study was to investigate the in vitro effect of crab shell extract on induction of apoptosis in breast cancer cell line (MCF7). Materials and Methods: Breast cancer cell line (MCF7) was used in this study. Crab shell was prepared and was powdered. Then the hydroalcoholic extracted was prepared and 6 concentrations (0, 100, 200, 400, 800 and 1000 μg /ml) and were affected on the cells within 72 hours. TUNEL method and fluorescent microscope were used to evaluate apoptosis of the cells. Data were analyzed by one way ANOVA and p<0.05 was considered significant. Results: Apoptosis Index of control, 100, 200, 400, 800 and 1000 μg/ml extracts were 1.85%, 3.55%, 4.91%, 10.62%, 17.55% and 21.48% respectively. 400, 800 and 1000 μg /ml concentration of extract had significant in comparison with control group (p<0.05). Conclusion: Crab shell extract induced apoptosis in breast cancer cells MCF7 dose-dependently.


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