Ovarian tissue and follicle culture

Hosseini, L.; Naderi, M.; Heidari, B.; Borjian Boroujeni, S.; Sarvari, A.; Behzadi, B.; Akhondi, M.; Shirazi, A.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p88
Academic Journal
Introduction: Currently, the culture of cryopreserved ovarian cortical tissue that contains large number of primordial follicles is a promising fertility preservation approach in young cancer patients. This option allows immediate initiation of cancer treatment and is the only suitable choice for pre-pubertal girls. To restore fertility using ovarian cortical strips, there are several choices depend on age and type of cancer. Materials and Methods: One of the several proposed approaches for fertility preservation in female cancer patients is ovarian tissue cryopreservation followed by transplantation, or ovarian tissue and follicles culture. Ovarian tissue can be transplanted when there is no risk of introducing malignant cells to the patient otherwise latter method should be considered. Therefore, to achieve complete development, multi-step culture system is more advocated. Results: For instance, the first few days ovarian tissue culture followed by culture of isolated follicles as 3-dimentional systems in the form of simple follicular culture or artificial ovary along with considering all required growth factors is received much attention. Due to complexity of factors and processes involved in follicular development and lack of enough knowledge about folliculogenesis at molecular and cellular levels, live birth has not been achieved, except in mouse, via follicular culture in mammals. Conclusion: Despite achieved progresses in assisted reproductive technologies, no reliable method for female fertility preservation using follicular culture is considered. Apart from 3-dimentional culture system, application of bioreactor besides all obtained progresses in the field of ovarian physiology and cryopreservation will be promising to this end.


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