Expression of trophectoderm specific gene, Fgf4 in artificially collapsed mouse blastocysts

Sehati, R.; Dashtizad, M.; Daliri, M.; Farokhi, F.; Fathalizadeh, P.; Zandi, G.; Gharib, S.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p88
Academic Journal
Introduction: In recent years, puncture of trophectoderm of expanded blastocyst before vitrification introduced a simple and effective technique to assist successful cryopreservation of expanded blastocyst by vitrification. Puncture of trophectoderm of expanded blastocyst prior to vitrification would allow more rapid re-expansion and enhance hatching rate. However, there is no guarantee on their normal development. Thus, we examined the effect of forced blastocoel collapse on viability and quality of embryos through expression of Fgf4 as a trophectoderm specific marker. Materials and Methods: In this experiment day 4 and day 5 mouse embryos were collected from synchronized mice and randomly divided into two groups. Expanded blastocysts in group 1 were artificially collapsed using ICSI pipette. Survival and hatching rate of collapsed blastocysts were assessed. Quantitative expression of Fgf4 was performed in these groups by Real-time PCR technique. Results: There was no degeneration of collapsed blastocysts. The proportion of blastocysts showing fast development tended to be higher when the blastocysts underwent AC. There was no clear correlation between morphology of AC embryos and control group and expression of Fgf4 significantly improved. Conclusion: Our results revealed that artificial collapse of blastocoele cavity can improve the survival and hatching rate of blastocysts before embryo transfer.


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