Quality assessment of blastocysts artificially collapsed by microneedle

Fathalizadeh, P.; Dashtizad, M.; Daliri Joupari, M.; Shamsara, M.; Asaadi Tehrani, G.; Zandi, G.; Rahim Tayefe, A.; Hajarian, H.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p87
Academic Journal
Introduction: Embryo cryopreservation is possible in all stages of pre-implantation, but according to some studies, the survival rate of cryopreserved blastocyst is lower than the other stages. This problem could be due to the fluid-filled cavity of blastocyst that provides a good ground for ice crystals formation and cell structure damages. Artificial collapse of blastocyst cavity is one of the assisted reproductive technologies that can diminish this problem by reducing the volume of blastocoelic fluid. But the risk of mechanical damages to blastomers is possible during this technique. The purpose of this study was to investigate the effect of artificial collapse of blastocyst on embryo quality through evaluation of Nanog gene expression that is effective in the pluripotency of inner cell mass. Materials and Methods: 3.5-4.5 days old In vivo mouse embryos were divided in treated and control groups randomly. Blastocysts were collapsed by using a microneedle in treated group. The Nanog gene expression assessed in treated group and fresh blastocysts as control group by Real-time PCR technique. Results: Nanog gene expression was increased in treated group in comparison with control group, but this increase was not statistically significant. Conclusion: Our results revealed that artificial collapse of blastocyst do not make undesired effects on Nanog gene expression.


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