Effect of in vitro fertilization on primitive endoderm specific genes expression in mouse blastocysts

Kazemi, P.; Dashtizad, M.; Ahangari, G.; Shamsara, M.; Mahdavinezhad, F.; Hajarian, H.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p85
Academic Journal
Introduction: It is generally accepted that mammalian preimplantation embryos are sensitive to their surrounding environment and culture conditions in case of in vitro studies. Culture conditions can affect gene expressions which are associated with embryo quality. Therefore, we compared the expression of primitive endoderm layer markers (Gata6, Grb2) and development of mouse blastocysts produced by in vitro fertilization (IVF) versus in vivo produced blastocysts. Materials and Methods: For IVF group, oocytes were isolated from the superovulated females and fertilized in vitro by capacitated sperm obtained from the cauda epididymis of male. The presumptive zygotes were cultured to the blastocyst stage. For control group, blastocysts were flushed out of the uterus on post mating day 3.5. Finally expression levels of Gata6 and Grb2 genes in both groups of blastocysts were analyzed by Real-time PCR. Results: In vitro blastocysts have a reduction in hatching rate compared with blastocysts generated in vivo (70.22% vs. 87.80%). Furthermore blastocysts produced by IVF had an increase in the expression levels of Gata6 and Grb2 when compared with the in vivo control embryos (p>0.05). Conclusion: Assisted hatching for in vitro produced embryos would be a useful approach to improve pregnancy outcome.


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