Effect of vitrification on expression of apoptotic specific genes, Bax and Bcl2 in IVF-derived versus in vivo embryos

Bolouri, R.; Dashtizad, M.; Shamsara, M.; Daliri Joupari, M.; Hashemi, M.; Fathalizadeh, P.; Zandi, G.; Hojjatzadeh Dezfuli, Z.; Hajarian, H.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p84
Academic Journal
Introduction: For embryo preservation in the long-term, cryopreservation is one of the undeniable technologies in assisted reproductive technologies. Exposure to a variety of cellular stressors, like culture media and high concentrations of cryoprotectants, can induce changes in gene expression and apoptosis pattern. This study compared vitrified-warmed in vivo and in vitro embryos in terms of viability and expression of Bax and Bcl2 genes which are involved in apoptosis. Materials and Methods: In experiment 1, about 300 in vivo blastocysts divided into 3 groups. Group A, vitrified/warmed blastocysts, group B, blastocysts just exposed to vitrification/warming solutions as toxicity test and finally group C, non-treated blastocysts as control. In experiment 2, same number of IVF-derived blastocysts divided into the same categories. Survival and hatching rates of blastocysts in all groups were assessed. Expression of Bax and Bcl2 was evaluated by Real Time PCR technique. Results: The proportion of hatching and hatched blastocysts in in-vivo blastocyst was notably higher than those observed in IVF-derived counterpart. No significant difference was observed in expression of Bax and Bcl2 genes between treated groups. Conclusion: These data suggest that vitrification of IVF-derived blastocysts can be used as an efficient method for blastocysts preservation.


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