Evaluation of MMP-2 gene expression in mice pre-antral follicles derived from vitrified and fresh ovarian tissue

Khosravi, S.; Zavareh, S.; Paylakhi, S.; Gorbanian, M.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p84
Academic Journal
Introduction: Cryopreservation is a useful method for preservation of ovarian tissue of patients who are candidate for chemo and radiotherapy. It has been postulated that MMP-2 participates in the process of remodeling of ovarian extracellular matrix such as the follicle growth and ovulation. The aim of this study was to compare the expression of MMP-2 gene of preantral follicles derived from vitrified ovaries with those derived from fresh ovaries. Materials and Methods: Ovaries of 14 to 16-days-old NMRI mice were randomly choose and evaluation of MMP-2 gene expression of preantral follicles isolated from vitrified ovarian tissue compared with fresh preantral follicles using Real time PCR. Preantral follicles with 140-160 μm in diameter were isolated from fresh and vitrified ovaries. Then, for evaluation of MMP-2 gene expression Real time PCR was used. Results: Expression of MMP-2 gene of preantral follicles isolated room vitrified ovaries was significantly higher than fresh preantral follicles. Conclusion: Vitrification of ovarian tissue causes increesed expression of MMP-2 of preantral follicles.


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