Effect of in vitro fertilization on expression of apoptotic specific gene in mouse embryos at blastocysts stage

Hojjatzadeh, Z.; Dashtizad, M.; Shamsara, M.; Hashemi, M.; Bolouri, R.; Zandi, G.; Fathalizadeh, P.; Hajarian, H.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p83
Academic Journal
Introduction: In vitro fertilization (IVF) is one of the assisted reproductive technologies used to help infertility treatment. In vitro culture of preimplantation embryos is associated with changes in gene expression. Exposure of preimplantation embryos to a variety of cellular stresses like culture media can induce apoptosis. It is however, not known if the method of fertilization affects the pattern of gene expression. We compared gene expression of IVF-derived mouse blastocysts versus in vivo produced blastocysts. The purpose of this study was to investigate the effect of in vitro fertilization on the expression of P53 gene which is involved in apoptosis process. Material and Methods: IVF-derived blastocysts were considered as group 1 and in vivo blastocysts as group 2. Expression of P53 gene in IVF and in vivo embryos were assessed with real-time PCR. Results: Our results showed no significant changes in the expression of P53 gene between two groups. Conclusion: In vitro fertilization can be used as an efficient and feasible method for infertility treatment.


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