TITLE

Effect of in vitro fertilization on expression of apoptotic specific gene in mouse embryos at blastocysts stage

AUTHOR(S)
Hojjatzadeh, Z.; Dashtizad, M.; Shamsara, M.; Hashemi, M.; Bolouri, R.; Zandi, G.; Fathalizadeh, P.; Hajarian, H.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p83
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: In vitro fertilization (IVF) is one of the assisted reproductive technologies used to help infertility treatment. In vitro culture of preimplantation embryos is associated with changes in gene expression. Exposure of preimplantation embryos to a variety of cellular stresses like culture media can induce apoptosis. It is however, not known if the method of fertilization affects the pattern of gene expression. We compared gene expression of IVF-derived mouse blastocysts versus in vivo produced blastocysts. The purpose of this study was to investigate the effect of in vitro fertilization on the expression of P53 gene which is involved in apoptosis process. Material and Methods: IVF-derived blastocysts were considered as group 1 and in vivo blastocysts as group 2. Expression of P53 gene in IVF and in vivo embryos were assessed with real-time PCR. Results: Our results showed no significant changes in the expression of P53 gene between two groups. Conclusion: In vitro fertilization can be used as an efficient and feasible method for infertility treatment.
ACCESSION #
96841688

 

Related Articles

  • Strategies for embryo utilization in assisted reproduction--how do we assess their relative.... Edgar, David H.; Speirs, Andrew L. // Journal of Assisted Reproduction & Genetics;Oct99, Vol. 16 Issue 9, p460 

    Examines the efficacy of assisted reproduction technology on embryo utilization. Impact of in vitro fertilization (IVF) on pregnancy rate; Blastocyst transfer as an option in human IVF; Rationale for an extended culture using a cell-free system; Efficiency of blastocyst freezing before an...

  • Sequential transfer of day 3 embryos and blastocysts after previous IVF failures despite adequate ovarian response. Machtinger, Ronit; Dor, Jehoshua; Margolin, Matbeii; Levron, Jacob; Baum, Micha; Ferber, Betty; Shulman, Adrian; Bider, David; Seidman, Daniel S. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Sep2006, Vol. 13 Issue 3, p376 

    The purpose of this study was to compare IVF outcome following sequential embryo transfer (ET) with that following the transfer of early cleavage embryos among patients with previous multiple IVF failures but adequate ovarian response. A retrospective matched case-control analysis was made of...

  • Picking winners. Conger, Krista // Stanford Medicine;Winter2011, Vol. 28 Issue 1, p6 

    The article presents a study on the technique which could predict whether a human embryo will develop successfully, within two days after its fertilization. The study uses time-lapse video and computer software in tracking the development of a subset of 242 frozen, one-cell human embryos from an...

  • Selection for faster development does not bias sex ratios resulting from blastocyst embryo transfer. Richter, K. S.; Anderson, M.; Osborn, B. H. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Apr2006, Vol. 12 Issue 4, p460 

    Concerns of possible male biased sex ratios with IVF exist due to reports of faster preimplantation development for male relative to female embryos in a variety of mammals, including humans. This study reports the first direct test of the hypothesis that selection for faster preimplantation...

  • Does early morphology provide additional selection power to blastocyst selection for transfer? Guerif, F.; Lemseffer, M.; Leger, J.; Bidault, R.; Cadoret, V.; Chavez, C.; Gasnier, O.; Saussereau, M. H.; Royere, D. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Oct2010, Vol. 21 Issue 4, p510 

    Routine early developmental parameters are widely used in IVF centres to evaluate embryo development and fresh single-blastocyst transfer currently seems superior to single-embryo transfer. Would early morphological parameters help to choose the single blastocyst to be transferred, thereby...

  • Pregnancy following a difficult transfer: a case report. Fonttis, A. Alosilla; Napolitano, R. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Jul/Aug2002, Vol. 5 Issue 1, p47 

    Provides information on a study that described a pregnancy obtained after transferring a blastocyst developed in a standard medium from a 72-h embryo, which had been retained on the catheter during a traumatic and bloody transfer. Methodology of the study; Results and discussion on the study.

  • Optimizing Culture Medium Greatly Improves Quality. Ogura, Atsuo // Fertility Weekly;9/25/2006, p2 

    The article reports on the research about the effect of the use of optimum culture medium on the quality of cumulus-free oocyte maturation in vitro. The use of TYH medium has resulted to a 92.3% maturation rate of oocytes. After in vitro fertilization or parthenogenetic activation, alphaMEM...

  • Cycle outcome can be predicted by the growth profile of untransferred cleavage-stage embryos. Yilmaz, S.; Kutlu, P.; Sofuoglu, K.; Delikara, N.; Kolankaya, A.; Arici, A. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Jan2008, Vol. 16 Issue 1, p124 

    Excess embryos obtained from intracytoplasmic sperm injection cycles may be cultured and observed until day 5 if the couple receiving treatment do not want them to be cryopreserved. In order to investigate the correlation between blastocyst formation in extended culture and pregnancy outcome,...

  • Pregnancy Rate Following Transfer of in vitro Produced Lamb Derived Embryos in Two Embryonic Stages. Shirazi, A.; Shams-Esfandabadi, N.; Ahmadi, E.; Jadidi, M.; Heidari, B. // Pakistan Journal of Biological Sciences;2008, Vol. 11 Issue 6, p938 

    Ovine embryos were produced by maturation, fertilization and in vitro culture (IVM/IVF/IVC) of oocytes collected from slaughtered prepubertal ewes. At 24 h post IVM, oocytes were fertilized with fresh semen collected from Lori-Bakhtiari breed at a concentration of 1.0x106 sperm mL-1. The...

  • The meaning of life--in the laboratory. Kass, Leon R. // Public Interest;Winter2002, Issue 146, p38 

    Examines whether the initiation and growth of human life in the laboratory should be encouraged or allowed. Circumstances of laboratory-grown blastocysts; Fates of the in vitro fertilized embryo; Main reason for bringing life into the laboratory; Arguments in favor of federal support for...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics