Mitochondria common deletion in cumulus cells in vitro fertilization-embryo transfer cycle

Fesahat, F.; Sheikhha, M. H.; Ebrahimi, B.; Halvaei, I.; Ashourzadeh, S.; Rasti, A.; Rastegar, M.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p82
Academic Journal
Introduction: The aim of this study was to investigate mitochondria DNA 4977bp deletion, known as mtDNA common deletion, in cumulus cells (CCs) and its probable relationship with patient's characteristics and in vitro fertilization outcomes. Material and Methods: A total 14 women undergoing IVF/ICSI were included the study.The CCs were collected during oocyte retrievals and harvested for DNA extraction. Human mtDNA common deletion was detected by multiplex PCRand gel electrophoresis. The relationship between mtDNA common deletion and etiology of infertility, patient'sage, embryoquality and pregnancy rates were analyzed accordingly. Embryo morphology was evaluated on day two based on Hill criteria. Results: The most cause of infertility was Male and tubal factors. The mean age of the patients was 30± 8. All of the embryos were fallen into A and B grades.No common deletion was detectedin the samples. This investigation is an ongoing study and our sample size would be 36 cases. The final results, which include probable relationship between common deletion and the aforementioned variables, will be presented at the congress. Conclusion: The result of this investigation may highlight the role of cumulus cells in oocyte evaluation and embryo selection in in vitro fertilization/embryo transfer cycles.


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