TITLE

Evaluation of in vitro effect of crab shell extract on nitric oxide production by prostate cancer cell line

AUTHOR(S)
Rezakhani, L.; Mirzapur, P.; Rashidi, Z.; Khazaei, M.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p81
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: Nitric oxide (NO) is a free radical and antioxidants which plays a pivotal role in many physiological and pathological processes such as angiogenesis, tumor cell growth and invasion. Antioxidants affect NO secretion by cells. Crab shell has various antioxidants. The aim of this study was to investigate nitric oxide levels of prostate cancer cells media treated with extracts of crab shell. Materials and Methods: Prostate cancer cell line (LNcap) was used in this study Crab shell was prepared and powdered, and then the hydroalcoholic extract was prepared. Five extracts concentrations (100, 200, 400, 800 and 1000 μg /ml) in 24, 48 and 72 hours were affected on the cells. NO levels were evaluated by Griess methods. Data were analyzed by one way ANOVA and P<0.05 was considered significant. Results: the effect of different concentrations of crab shell extract on LNcap cell line in 24, 48, and 72-hour periods indicated decreasing NO secretion in dose and time dependant manner. The 1000μg/ml dose has significant difference compared to control group (p⩽0.05). Conclusion: Hydroalcoholic extract of Crab shell reduced nitric oxide secretion by prostate cancer cells (LNcap) in dose and time-dependent manner.
ACCESSION #
96841682

 

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