Evidence for dynamic role of NFY in cellular processes related to endometriosis

Ashini, M.; Shahhoseini, M.; Mahdian, S.; Moeeni, A.; Shiva, M.; Ramezanali, F.; Ashrafi, M.; Aflatoonian, R.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p69
Academic Journal
Introduction: Nuclear factor-Y (NFY) is the major CCAAT-binding transcription factor that has a crucial function in transcriptional regulation of cell cycle genes. This hetrotrimeric protein has three different subunits including NFY-A, B and C, which are all necessary for binding to DNA with the high specificity and affinity. Due to the high cell proliferation in endometrium during menstrual cycle and aberrant endometrial changes during endometriosis, it seems that this transcription factor may be involved in occurrence characteristics of endometriosis. Materials and Methods: The aim of this study was to compare expression profile of NFY coding gene by realtime PCR. For this respect, 26 laparoscopically confirmed endometriosis patients, parallel with 18 control samples were collected from healthy fertile women, between 20-45 years old, undergoing tubal ligation surgery. Ethical approval and informed patient consent was gained for the use of tissue samples. Results: Our results revealed that the mRNA level of all subunits of NFY showed significant higher expression in ectopic samples during secretory and proliferative phase, compare to control group. Also, in eutopic samples, the expression level of these genes, except for NFY-B in secretory phase and NFY-C in proliferative phase, were higher than the control group. Conclusion: These results suggest the dynamic role of NFY in endometriosis, maybe through induction of proliferation of endometrial cells, especially in occurrence of ectopic endometriosis.


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