Relationship between sperm parameters and DNA fragmentation using Halosperm kit

Javadi, M.; Ratighdoost, H.; Farsi, M.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p68
Academic Journal
Introduction: Sperm DNA integrity is the most important part for transferring the genetic information. Sperm DNA fragmentation is considered as male infertility and its assessment completed conventional semen analysis and it's emphasized to evaluate it in fertility clinics. In this study, we investigated the relationship between sperm parameters) motility, morphology, concentration) and DNA fragmentation level of sperm (DFLs) Materials and Methods: 89 infertile men were assessed by conventional analysis of sperm and DFLs determination by Halosperm which is sperm chromatin dispersion test (SCD), this assessment was done in two groups of patients, low DNA fragmentation group (LFG <30%) and high DNA fragmentation group (HFG >30%). Results: Sperm parameters (concentration, motility and morphology) in HFG was significantly lower than LF group (pO.OOl, pO.OOl, and pO.Ol, respectively) after preparation. Logistic regression model showed morphology and motility variables were the predictors of DFLs, and cut off points of them were 5% and 50%. Conclusion: Our results show a negative relationship between DFLs and sperm parameters after preparation. Also, according to DF>30% in percentage of men with normal semen parameters the high importance of assessment DF test in clinics in order to determine sperm DNA problems and discovering sperm abnormality in the patients is sensed.


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