TITLE

The vascular endothelial growth factor VEGF+405 G/G genotype may influence embryo implantation in assisted reproductive techniques

AUTHOR(S)
Rokhsattalab, Z.; Gourabi, H.; Hasanzad, M.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p68
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: Repeated implantation failure (RIF) is the main problem after using assisted reproductive techniques (ART). The main causes of RIF as a multifactorial problem include decrease in endometrial receptivity, defects of embryo or combinational. Successful embryo implantation depends on trophoblast proliferation, migration and invasion to the endometrium, all associated with vascular endothelial growth factor (VEGF) as the major protein in stimulating of angiogenesis. This study aimed to determine the association betweenVEGF+405G/C polymorphism and RIF in infertile women. Materials and Methods: The patients group included 74 women with >3 RIF and the control group consisted of 149 healthy fertile women. Genotypes and allele frequencies of VEGF+405G/C polymorphism were determined by PCR-RFLP method and verified by Sanger sequencing. Results: The frequencies of GG, GC and CC genotypes in patients group were 31.1%, 48.6% and 20.3%, respectively while those frequencies in controls were 2.0%, 47.0% and 51.0% respectively. The frequency of GG genotype was significantly higher in patients than controls (p<0.001). CC genotype frequency was higher in controls than patients (pO.OOl). The frequency of GC genotype did not show any difference between groups. C as the wild allele was more frequent in controls while frequency of G as the mutant allele was higher in patients (pO.OOl). Conclusion: The VEGF+405 G/G genotype may influence embryo implantation and lead to RIF in ART candidates. Since this is the second report on association of this polymorphism with RIF, further studies in different ethnic populations require for determining this association.
ACCESSION #
96841646

 

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