Epigenetic pattern of HOXA10 gene in human endometrium during menstrual cycle

Samadieh, Y.; Shahhoseini, M.; Favaedi, R.; Hosseini, E.; Ramezanali, F.; Ashrafi, M.; Aflatoonian, R.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p67
Academic Journal
Introduction: Epigenetic pattern of HOXA10, as a key gene responsible for uterine organogenesis, functional endometrial differentiation and endometrial receptivity, have not been fully characterized. The aim of this study is to investigate the epigenetic regulation of HOXA 10 promoter and its correlation with mRNA expression of this gene in endometrial tissue, during menstrual cycle. Materials and Methods: Endometrial tissues were collected from 18 healthy fertile women undergoing laparoscopy for tubal ligation surgery. Ethical approval and informed patient consent was gained for the use of tissue samples. Quantitative expression analysis was performed by real-time PCR technique and epigenetic analysis was performed by Chromatin Immunoprecipitation (ChIP), using anti- H3K9ac, MeCP2, H3K9Me2, H3K27Me3, H3K4Me3 antibodies. Results: Our results showed a correlation between HOXA10 mRNA expression and epigenetic marks of its promoter, during menstrual cycle. In the way that, H3K9ac and H3K4Me3, known to be associated with gene activation, were significantly higher during secretory phase of menstrual cycle in comparison to proliferative phase. In contrast, H3K9Me2, H3K.27Me3 and MeCP2 marks, known to be associated with gene repression, were significantly higher during proliferative phase in comparison to secretory phase of menstrual cycle. Conclusion: This study provides support for a possible role of histone codes and epigenetic marks, in regulation of mRNA expression of HOXA10 during menstrual cycle. Our findings indicate that HOXAIO can be noted as a candidate gene which its aberrant expression and histone modification may contribute to the etiology of infertility and other gynecological disorders.


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