Cytosolic and mitochondrial ROS: which one is associated with poor chromatin remodeling?

Bahrami, S.; Kiani-Esfahani, A.; Tavalaee, M.; Deemeh, M.; Nasr-Esfahani, M.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p66
Academic Journal
Introduction: The aim of this study was to determine if there are any associations between impaired chromatin packaging and the origin of reactive oxygen species (ROS) production. Materials and Methods: Cytosolic ROS, mitochondrial ROS, DNA protamination, and apoptosis were evaluated with dichlorofluoresce in diacetate (DCFH-DA), dihydrorhodamine 123 (DHR123), chromomycin A3 (CMA3), and YO-Pro-1 (Yl)/propidium iodide (PI), respectively, by flow cytometry (FCM) in 40 infertile individuals. Results: Percentages DCF+ and R123+ sperm were positively associated with percentage CMA3+ sperm and negatively associated with percentage apoptotic sperm. No correlation was observed between CMA3+ sperm and the percentage of apoptotic sperm. Conclusion: Under protamination of sperm is not associated with the origin of ROS production, but their relationship may suggest an association with general physiological dysfunction of sperm. Furthermore, under protamination does make sperm prone to apoptosis. Rather, it is likely that apoptosis is induced by ROS production. Considering that these conclusions are derived from correlative analyses, additional studies including an interventive approach are required.


Related Articles

  • M2 Thawing Method has Positive Effect on Sperm Parameters. Gadea, Joaquin // Fertility Weekly;9/3/2012, p4 

    The article presents a study which aims to compare the impacts of two thawing processes on the functionality of the thawed spermatozoa. Analysis was performed on the impact of the thawing process on sperm chromatin condensation, level of reactive oxygen species (ROS) and sperm viability and...

  • Relations between clusters of oxidatively damaged nucleotides and active or open nucleosomes in the rat Nth 1 gene. Nomoto, Minoru; Yamaguchi, Raizo; Kohno, Kimitoshi; Kasai, Hiroshi // Oncogene;3/7/2002, Vol. 21 Issue 11, p1649 

    Presents a study that examined the molecular mechanism of the restricted formation of damaged nucleotides. Background on the kinds of DNA damage induced by reactive oxygen species; Importance of litigation-mediated polymerase chain reaction in the detection of damaged nucleotides; Impact of...

  • Proteomic identification of early salicylate- and flg22-responsive redox-sensitive proteins in Arabidopsis. Liu, Pei; Zhang, Huoming; Yu, Boying; Xiong, Liming; Xia, Yiji // Scientific Reports;2/27/2015, p8625 

    Accumulation of reactive oxygen species (ROS) is one of the early defense responses against pathogen infection in plants. The mechanism about the initial and direct regulation of the defense signaling pathway by ROS remains elusive. Perturbation of cellular redox homeostasis by ROS is believed...

  • Molecular determinants of nucleosome retention at CpG-rich sequences in mouse spermatozoa. Erkek, Serap; Hisano, Mizue; Liang, Ching-Yeu; Gill, Mark; Murr, Rabih; Dieker, Jürgen; Schübeler, Dirk; Vlag, Johan van der; Stadler, Michael B; Peters, Antoine H F M // Nature Structural & Molecular Biology;Jul2013, Vol. 20 Issue 7, p868 

    In mammalian spermatozoa, most but not all of the genome is densely packaged by protamines. Here we reveal the molecular logic underlying the retention of nucleosomes in mouse spermatozoa, which contain only 1% residual histones. We observe high enrichment throughout the genome of nucleosomes at...

  • Decreased expression levels of PRM1 and PRM2 genes in testes of infertile men referred to Royan Institute. Eelaminejad, Z.; Heaydaryan, N.; Favaedi, R.; Sadighi Gilani, M. A.; Shahhoseini, M. // Iranian Journal of Reproductive Medicine;AprSupp, p39 

    Introduction: Spermatogenesis is a complex and unique process in male reproductive system which transforms diploid spermatogonia into differentiated haploid spermatozoa. The final stage of this process needs extensive chromatin condensation. This condensation occurs by replacement of histones by...

  • Differential Gene Susceptibility to Sperm DNA Damage: Analysis of Developmental Key Genes in Trout. González-Rojo, Silvia; Fernández-Díez, Cristina; Guerra, Susana M.; Robles, Vanesa; Herraez, Maria Paz // PLoS ONE;Dec2014, Vol. 9 Issue 12, p1 

    Sperm chromatin in mammals is packaged in different blocks associated to protamines (PDNA), histones (HDNA), or nuclear matrix proteins. Differential packaging has been related to early or late transcription and also to differential susceptibility to genotoxic damage. Genes located in the more...

  • Cloning and Functional Analysis of Histones H3 and H4 in Nuclear Shaping during Spermatogenesis of the Chinese Mitten Crab, Eriocheir sinensis. Wu, Jiang-Li; Kang, Xian-Jiang; Guo, Ming-Shen; Mu, Shu-Mei; Zhang, Zhao-Hui // PLoS ONE;May2015, Vol. 10 Issue 5, p1 

    During spermatogenesis in most animals, the basic proteins associated with DNA are continuously changing and somatic-typed histones are partly replaced by sperm-specific histones, which are then successively replaced by transition proteins and protamines. With the replacement of sperm nuclear...

  • Protamine contents and P1/P2 ratio in human spermatozoa from smokers and non-smokers. Hammadeh, M. E.; Hamad, M. F.; Montenarh, M.; Fischer-Hammadeh, C. // Human Reproduction;Nov2010, Vol. 25 Issue 11, p2708 

    Background: Protamine content is necessary for proper sperm chromatin condensation and subsequent male fertility. The exact effect of smoking on male fertility remains controversial. The objective of this study was to evaluate the effect of smoking on protamine content of sperm in...

  • Relationship between reactive oxygen species production in human semen and sperm DNA damage assessed by Sperm Chromatin Structure Assay. Novotny, Jiri; Aziz, Nabil; Rybar, Roman; Brezinova, Jana; Kopecka, Vera; Filipcikova, Radka; Reruchova, Marie; Oborna, Ivana // Biomedical Papers of the Medical Faculty of Palacky University i;Dec2013, Vol. 157 Issue 4, p383 

    Aim. The aim of this prospective study was to find possible relationship between ROS production measured by chemiluminescence and flow cytometry in human semen and sperm DNA damage estimated by Sperm Chromatin Structure Assay. Methods. Study included 39 men from infertile couples and 23 fertile...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics