Global epigenetic modification profile of endometrial and endometriotic tissue shows significant alterations in endometriosis

Hosseini, E.; Shahhoseini, M.; Favaedi, R.; Samadieh, Y.; Ramazanali, F.; Afsharian, P.; Aflatoonian, R
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p65
Academic Journal
Introduction: Endometriosis is found in 2-8% of the general population but is present in 25-40% of women with infertility. Although endometriosis is a multifactorial disease and the exact etiology is not clearly understood, recently, it has been confirmed that epigenetic is associated with the molecular features of endometriosis. Two main epigenetic regulatory mechanisms, DNA methylation and histone modifications recognize the states of diseases. Therefore, the aim of this study was to investigate alterations of DNA methylation and histone acetylation/methylation levels in eutopic and ectopic endometrium of endometriosis patients. Materials and Methods: Eutopic and ectopic endometrium samples (n=5) were collected from endometriosis patients undergoing surgery and biopsy, as well as endometrial tissues from healthy fertile women (n=5). Chromatin extracts from samples were prepared following fixation and then shearing into fragments by sonication. Nucleosome ELISA was performed on chromatin extracts, in order to identify Global histone H3K9 acetylation/methylation and DNA methylation, using antibodies against H3K9ac, H3K9me and MeCP2, respectively. Results: We have identified global histone H3K9 hypermethylation in ectopic and eutopic endometrium, compared with controls. A significant hyperacetylation at histone H3K9 was observed in eutopic samples compared to ectopic and control groups. Furthermore, eutopic endometrial samples were globally DNA hypermethylated in comparison with controls. Conclusion: These results clearly show an epigenetic switch in endometrial and endometriotic tissue of patients with endometriosis, in the way that aberrant DNA methylation and histone acetylation/methylation status may play a dynamic role in occurrence of endometriosis and support the opinion that epigenetic abnormalities have causative functions in endometriosis.


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