A review of sperm preparation techniques; advantages and disadvantages

Neamati, N.; Aliyari, M.; Mohammadzadeh, J.; Shahsavan, M.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p65
Academic Journal
Introduction : Intrauterine insemination (IUI) with or without controlled ovarian hyperstimulation (COH+IUI) is the first choice to treatment of infertility in the cases of mild or moderate male subfertility and female with mild or moderate endometriosis and some types of ovulatory dysfunction. Materials and Methods: We reviewed texts and published articles since 2000 till 2014 about IUI and related subjects to determine the best practical techniques for preparation of high quality sperm sample to insemination. Results: Based on semen sample characteristics we can choose one of the standard sperm wash (SSW), Swim-Up (SWU), Swim-Down (SWD), or Density Gradient Centrifugation (DGC) methods according to standard and Good Laboratory Practice (GLP) guidelines to prepare efficient and potent sperm sample for IUI or other assisted reproductive techniques Conclusion: Each of the preparation techniques that mentioned has its own advantaged and disadvantages; clearly indicates that an exact and careful selection of proper preparation technique can improve the success of insemination.


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